Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (186K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (124)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Leone, G.
Right arrow Articles by Schoen, C. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leone, G.
Right arrow Articles by Schoen, C. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 26, Issue 9 2150-2155, Copyright © 1998 by Oxford University Press

ARTICLES

Molecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA

G Leone, H van Schijndel, B van Gemen, FR Kramer and CD Schoen
DLO Research Institute for Plant Protection (IPO-DLO), PO Box 9060, 6700 GW Wageningen, The Netherlands. leone@ipo.dlo.nl

Molecular beacon probes can be employed in a NASBA amplicon detection system to generate a specific fluorescent signal concomitantly with amplification. A molecular beacon, designed to hybridize within the target sequence, was introduced into NASBA reactions that amplify the genomic RNA of potato leafroll virus (PLRV). During amplification, the probe anneals to the antisense RNA amplicon generated by NASBA, producing a specific fluorescent signal that can be monitored in real- time. The assay is rapid, sensitive and specific. As RNA amplification and detection can be carried out in unopened vessels, it minimizes the risk of carry-over contaminations. Robustness has been verified on real- world samples. This homogeneous assay, called AmpliDet RNA, is a significant improvement over current detection methods for NASBA amplicons and is suitable for one-tube applications ranging from high- throughput diagnostics to in vivo studies of biological activities.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
A. Lasham, M. Herbert, N. Coppieters 't Wallant, R. Patel, S. Feng, M. Eszes, H. Cao, and G. Reid
A rapid and sensitive method to detect siRNA-mediated mRNA cleavage in vivo using 5' RACE and a molecular beacon probe
Nucleic Acids Res., November 26, 2009; (2009) gkp1076v1.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
M. B. Kerby, S. Freeman, K. Prachanronarong, A. W. Artenstein, S. M. Opal, and A. Tripathi
Direct Sequence Detection of Structured H5 Influenza Viral RNA
J. Mol. Diagn., May 1, 2008; 10(3): 225 - 235.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. J. Li, Y. Chu, B. Y.-H. Lee, and X. S. Xie
Enzymatic signal amplification of molecular beacons for sensitive DNA detection
Nucleic Acids Res., April 1, 2008; 36(6): e36 - e36.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
W. van der Meide, J. Guerra, G. Schoone, M. Farenhorst, L. Coelho, W. Faber, I. Peekel, and H. Schallig
Comparison between Quantitative Nucleic Acid Sequence-Based Amplification, Real-Time Reverse Transcriptase PCR, and Real-Time PCR for Quantification of Leishmania Parasites
J. Clin. Microbiol., January 1, 2008; 46(1): 73 - 78.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
K. Loens, T. Beck, D. Ursi, M. Overdijk, P. Sillekens, H. Goossens, and M. Ieven
Development of Real-Time Multiplex Nucleic Acid Sequence-Based Amplification for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp. in Respiratory Specimens
J. Clin. Microbiol., January 1, 2008; 46(1): 185 - 191.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
E. M. Fykse, G. Skogan, W. Davies, J. S. Olsen, and J. M. Blatny
Detection of Vibrio cholerae by Real-Time Nucleic Acid Sequence-Based Amplification
Appl. Envir. Microbiol., March 1, 2007; 73(5): 1457 - 1466.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
N. Mehra, M. Penning, J. Maas, L. V. Beerepoot, N. van Daal, C. H. van Gils, R. H. Giles, and E. E. Voest
Progenitor Marker CD133 mRNA Is Elevated in Peripheral Blood of Cancer Patients with Bone Metastases.
Clin. Cancer Res., August 15, 2006; 12(16): 4859 - 4866.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Yi, W. Zhang, and D. Y. Zhang
Molecular Zipper: a fluorescent probe for real-time isothermal DNA amplification
Nucleic Acids Res., July 5, 2006; 34(11): e81 - e81.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
K. Loens, T. Beck, H. Goossens, D. Ursi, M. Overdijk, P. Sillekens, and M. Ieven
Development of Conventional and Real-Time Nucleic Acid Sequence-Based Amplification Assays for Detection of Chlamydophila pneumoniae in Respiratory Specimens
J. Clin. Microbiol., April 1, 2006; 44(4): 1241 - 1244.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
K. H. Abd El Galil, M. A. El Sokkary, S. M. Kheira, A. M. Salazar, M. V. Yates, W. Chen, and A. Mulchandani
Real-Time Nucleic Acid Sequence-Based Amplification Assay for Detection of Hepatitis A Virus
Appl. Envir. Microbiol., November 1, 2005; 71(11): 7113 - 7116.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
M. Parida, K. Horioke, H. Ishida, P. K. Dash, P. Saxena, A. M. Jana, M. A. Islam, S. Inoue, N. Hosaka, and K. Morita
Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real-Time Reverse Transcription-Loop-Mediated Isothermal Amplification Assay
J. Clin. Microbiol., June 1, 2005; 43(6): 2895 - 2903.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Horejsh, F. Martini, F. Poccia, G. Ippolito, A. Di Caro, and M. R. Capobianchi
A molecular beacon, bead-based assay for the detection of nucleic acids by flow cytometry
Nucleic Acids Res., January 19, 2005; 33(2): e13 - e13.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
D. Rodriguez-Lazaro, M. D'Agostino, M. Pla, and N. Cook
Construction Strategy for an Internal Amplification Control for Real-Time Diagnostic Assays Using Nucleic Acid Sequence-Based Amplification: Development and Clinical Application
J. Clin. Microbiol., December 1, 2004; 42(12): 5832 - 5836.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
M. Parida, G. Posadas, S. Inoue, F. Hasebe, and K. Morita
Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification for Rapid Detection of West Nile Virus
J. Clin. Microbiol., January 1, 2004; 42(1): 257 - 263.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Rajendran and A. D. Ellington
In vitro selection of molecular beacons
Nucleic Acids Res., October 1, 2003; 31(19): 5700 - 5713.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. Mao, C. Luo, and Q. Ouyang
Studies of temperature-dependent electronic transduction on DNA hairpin loop sensor
Nucleic Acids Res., September 15, 2003; 31(18): e108 - e108.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
K. Loens, M. Ieven, D. Ursi, T. Beck, M. Overdijk, P. Sillekens, and H. Goossens
Detection of Mycoplasma pneumoniae by Real-Time Nucleic Acid Sequence-Based Amplification
J. Clin. Microbiol., September 1, 2003; 41(9): 4448 - 4450.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Tsourkas, M. A. Behlke, S. D. Rose, and G. Bao
Hybridization kinetics and thermodynamics of molecular beacons
Nucleic Acids Res., February 15, 2003; 31(4): 1319 - 1330.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
A. J. Lambert, D. A. Martin, and R. S. Lanciotti
Detection of North American Eastern and Western Equine Encephalitis Viruses by Nucleic Acid Amplification Assays
J. Clin. Microbiol., January 1, 2003; 41(1): 379 - 385.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. J. A. M. Weusten, P. A. W. M. Wouters, M. C. A. van Zuijlen, and P. A. van de Wiel
Stochastic processes defining sensitivity and variability of internally calibrated quantitative NASBA-based viral load assays
Nucleic Acids Res., December 15, 2002; 30(24): e137 - e137.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. Nutiu and Y. Li
Tripartite molecular beacons
Nucleic Acids Res., September 15, 2002; 30(18): e94 - e94.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. J. A. M. Weusten, W. M. Carpay, T. A. M. Oosterlaken, M. C. A. van Zuijlen, and P. A. van de Wiel
Principles of quantitation of viral loads using nucleic acid sequence-based amplification in combination with homogeneous detection using molecular beacons
Nucleic Acids Res., March 15, 2002; 30(6): e26 - e26.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
R. S. Lanciotti and A. J. Kerst
Nucleic Acid Sequence-Based Amplification Assays for Rapid Detection of West Nile and St. Louis Encephalitis Viruses
J. Clin. Microbiol., December 1, 2001; 39(12): 4506 - 4513.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
N. W. SCHLUGER
Changing Approaches to the Diagnosis of Tuberculosis
Am. J. Respir. Crit. Care Med., December 1, 2001; 164(11): 2020 - 2024.
[Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
H. H. El-Hajj, S. A. E. Marras, S. Tyagi, F. R. Kramer, and D. Alland
Detection of Rifampin Resistance in Mycobacterium tuberculosis in a Single Tube with Molecular Beacons
J. Clin. Microbiol., November 1, 2001; 39(11): 4131 - 4137.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
S. Yates, M. Penning, J. Goudsmit, I. Frantzen, B. van de Weijer, D. van Strijp, and B. van Gemen
Quantitative Detection of Hepatitis B Virus DNA by Real-Time Nucleic Acid Sequence-Based Amplification with Molecular Beacon Detection
J. Clin. Microbiol., October 1, 2001; 39(10): 3656 - 3665.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
C. Reinbold, F. E. Gildow, E. Herrbach, V. Ziegler-Graff, M. C. Goncalves, J. F. J. M. van den Heuvel, and V. Brault
Studies on the role of the minor capsid protein in transport of Beet western yellows virus through Myzus persicae
J. Gen. Virol., August 1, 2001; 82(8): 1995 - 2007.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Bernacchi and Y. Mely
Exciton interaction in molecular beacons: a sensitive sensor for short range modifications of the nucleic acid structure
Nucleic Acids Res., July 1, 2001; 29(13): e62 - e62.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
M. P. de Baar, M. W. van Dooren, E. de Rooij, M. Bakker, B. van Gemen, J. Goudsmit, and A. de Ronde
Single Rapid Real-Time Monitored Isothermal RNA Amplification Assay for Quantification of Human Immunodeficiency Virus Type 1 Isolates from Groups M, N, and O
J. Clin. Microbiol., April 1, 2001; 39(4): 1378 - 1384.
[Abstract] [Full Text]

Home page
 Genes Dev.Home page
D. Berger and T. Altmann
A subtilisin-like serine protease involved in the regulation of stomatal density and distribution in Arabidopsis thaliana
Genes & Dev., May 1, 2000; 14(9): 1119 - 1131.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. A. M. Vet, A. R. Majithia, S. A. E. Marras, S. Tyagi, S. Dube, B. J. Poiesz, and F. R. Kramer
Multiplex detection of four pathogenic retroviruses using molecular beacons
PNAS, May 25, 1999; 96(11): 6394 - 6399.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.