Nucleic Acids Research, Vol 26, Issue 9 2161-2167, Copyright © 1998 by Oxford University Press
G Fu and DC Barker
Characterisation of the telomeres of Leishmania is important for
understanding many aspects of the parasitic life of this primitive
protozoan and for the completion of the physical map and sequencing of the
genome. After sequencing more than 300 telomere-derived clones from
Leishmania braziliensis and Leishmania major, a conserved 100 bp sequence
was identified immediately adjacent to the telomere at the chromosome end
and was named LCTAS (Leishmania conserved telomere- associated sequence).
The LCTAS contains two conserved sequence boxes, and is present in all
Leishmania species studied. The organisation of the LCTAS in the telomeric
region differs between L. braziliensis and L. major: in L. major the LCTASs
are tandemly repeated, while in L. braziliensis the LCTAS is present as a
single copy per end. Two additional TASs with 1.6 kb and 274 bp repeat
structures, which are apparently different to LCTAS, were isolated and
mapped onto a L. braziliensis 250 kb multicopy minichromosome and the L.
major chromosome 1, respectively. An unusual feature in L. braziliensis is
that the telomeric repeats are often comprised of a novel tandem repeat
CCCTAACCCGTGGA. A 'slippage' mechanism for LCTAS formation is proposed in
this study as an alternative way for the synthesis and maintenance of
telomeres and subtelomere regions.
ARTICLES
Characterisation of Leishmania telomeres reveals unusual telomeric repeats and conserved telomere-associated sequence
MRC Outstation of NIMR, Molteno Laboratories, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK.
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