Nucleic Acids Research, Vol 27, Issue 13 2569-2577, Copyright © 1999 by Oxford University Press
TJ Parry, C Cushman, AM Gallegos, AB Agrawal, M Richardson, LE Andrews, L Maloney, VR Mokler, FE Wincott and PA Pavco
Vascular endothelial growth factor (VEGF) and its receptors Flt-1 and KDR
play important roles in physiological and pathological angiogenesis.
Ribozymes that target the VEGF receptor mRNAs were developed and their
biological activities in cell culture and an animal model were assessed.
Ribozymes targeting Flt-1 or KDR mRNA sites reduced VEGF-induced
proliferation of cultured human vascular endothelial cells and specifically
lowered the level of Flt-1 or KDR mRNA present in the cells. Anti- Flt-1
and KDR ribozymes also exhibited anti-angiogenic activity in a rat corneal
pocket assay of VEGF-induced angiogenesis. This report illustrates the
anti-angiogenic potential of these ribozymes as well as their value in
studying VEGF receptor function in normal and pathophysiologic states.
ARTICLES
Bioactivity of anti-angiogenic ribozymes targeting Flt-1 and KDR mRNA
Ribozyme Pharmaceuticals, Inc., 2950 Wilderness Place, Boulder, CO 80301, USA and.
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