Nucleic Acids Research, Vol 27, Issue 13 2585-2590, Copyright © 1999 by Oxford University Press
PG Zaphiropoulos
Reverse transcription-PCR analysis in human epidermis, using primers from
CYP2C18 and CYP2C19, revealed products containing combinations between
canonically defined exons of these two genes. The major RNA species
identified contained 2C18 exon 8 spliced with 2C19 exon 2. However, the
terminal exons 1 and 9 were never detected in any of these composite
molecules. When similar experiments were performed with liver RNA, exons 1
and 9 of both 2C18 and 2C19 were readily identified in composite 2C18/2C19
RNAs. Moreover, molecules containing 2C9 sequences spliced with 2C18 exons
were also detected. These findings suggest that during the process of RNA
splicing of the 2C transcripts, various exon juxtaposition events may
occur, including combinations between exons of distinct genes. However, the
frequency of these events is quite low and the levels of the composite RNA
molecules are generally estimated at less than one molecule per cell. Since
the order of these genes on chromosome 10q24 is CYP2C18 - CYP2C19 - CYP2C9,
it is conceivable that the composite RNAs may result from multiple
canonical and inverse splicing events of a long pre-mRNA that encompasses
the three genes. However, these molecules could also be rationalized as
being the products of trans splicing phenomena between distinct pre-mRNAs.
ARTICLES
RNA molecules containing exons originating from different members of the cytochrome P450 2C gene subfamily (CYP2C) in human epidermis and liver
Department of Bioscience, Center for Nutrition and Toxicology, Karolinska Institute, Novum, 141 57 Huddinge, Sweden. peza@cnt.ki.se
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