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Nucleic Acids Research, Vol 27, Issue 13 2644-2645, Copyright © 1999 by Oxford University Press

ARTICLES

BseSI, a restriction endonuclease from Bacillus stearothermophilus Jo 10-553, which recognizes the novel hexanucleotide sequence 5'- G(G/T)GC(A/C)C-3'

D Steponaviciene, Z Maneliene, M Petrusyte and A Janulaitis
MBI Fermentas, Institute of Biotechnology, Graiciuno 8, 2028 Vilnius, Lithuania.

A new restriction endonuclease Bse SI has been isolated from Bacillus stearothermophilus Jo10-553. Bse SI recognizes a degenerate hexanucleotide sequence 5'-G(G/T)GC(A/C)C-3' and cleaves DNA to produce 3[prime]-protruding tetranucleotide ends.
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