Nucleic Acids Research, Vol 27, Issue 15 3130-3137, Copyright © 1999 by Oxford University Press
K Tanimoto, Q Liu, J Bungert and JD Engel
The polyoma virus enhancer (PyE) is capable of conferring integration
position-independent expression to linked genes in stably transfected
erythroid cells after joining to DNase I hypersensitive site (HS) 5 of the
human beta-globin locus control region (LCR). In attempting to separate the
chromatin opening activity of the LCR from its enhancer activity and to
investigate contributions of the individual HS core elements to LCR
function, the human beta-globin LCR HS2, HS3 and HS4 core elements were
replaced with the PyE within the context of a yeast artificial chromosome
(YAC) bearing the whole locus. We show here that, in contrast to its
function in cultured cells, the PyE is unable to replace HS core element
function in vivo. We found that the PyE substitution mutant LCR is unable
to provide either chromatin opening or transcriptional potentiating
activity at any erythroid developmental stage in transgenic mice. These
data provide direct evidence that the human beta-globin LCR core elements
specify unique functions that cannot be replaced by a ubiquitous enhancer
activity.
ARTICLES
The polyoma virus enhancer cannot substitute for DNase I core hypersensitive sites 2-4 in the human beta-globin LCR
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208-3500, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  X. Fang, J. Sun, P. Xiang, M. Yu, P. A. Navas, K. R. Peterson, G. Stamatoyannopoulos, and Q. Li Synergistic and Additive Properties of the Beta-Globin Locus Control Region (LCR) Revealed by 5'HS3 Deletion Mutations: Implication for LCR Chromatin Architecture Mol. Cell. Biol., August 15, 2005; 25(16): 7033 - 7041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Omori, O. Tanabe, J. D. Engel, A. Fukamizu, and K. Tanimoto Adult Stage {gamma}-Globin Silencing Is Mediated by a Promoter Direct Repeat Element Mol. Cell. Biol., May 1, 2005; 25(9): 3443 - 3451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tanimoto, A. Sugiura, A. Omori, G. Felsenfeld, J. D. Engel, and A. Fukamizu Human {beta}-Globin Locus Control Region HS5 Contains CTCF- and Developmental Stage-Dependent Enhancer-Blocking Activity in Erythroid Cells Mol. Cell. Biol., December 15, 2003; 23(24): 8946 - 8952. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. C. Ostermeier, Z. Liu, R. P. Martins, R. R. Bharadwaj, J. Ellis, S. Draghici, and S. A. Krawetz Nuclear matrix association of the human {beta}-globin locus utilizing a novel approach to quantitative real-time PCR Nucleic Acids Res., June 15, 2003; 31(12): 3257 - 3266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Harju, K. J. McQueen, and K. R. Peterson Chromatin Structure and Control of {beta}-Like Globin Gene Switching Experimental Biology and Medicine, October 1, 2002; 227(9): 683 - 700. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tanimoto, Q. Liu, F. Grosveld, J. Bungert, and J. D. Engel Context-dependent EKLF responsiveness defines the developmental specificity of the human varepsilon -globin gene in erythroid cells of YAC transgenic mice Genes & Dev., November 1, 2000; 14(21): 2778 - 2794. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Lamartina, E. Sporeno, E. Fattori, and C. Toniatti Characteristics of the Adeno-Associated Virus Preintegration Site in Human Chromosome 19: Open Chromatin Conformation and Transcription-Competent Environment J. Virol., August 15, 2000; 74(16): 7671 - 7677. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. E. Rubin, P. Pasceri, X. Wu, P. Leboulch, and J. Ellis Locus control region activity by 5'HS3 requires a functional interaction with beta -globin gene regulatory elements: expression of novel beta /gamma -globin hybrid transgenes Blood, May 15, 2000; 95(10): 3242 - 3249. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Wei and M. D. Brennan Polarity of transcriptional enhancement revealed by an insulator element PNAS, December 19, 2000; 97(26): 14518 - 14523. [Abstract] [Full Text] [PDF]
|
|