Nucleic Acids Research, Vol 27, Issue 15 3153-3158, Copyright © 1999 by Oxford University Press
LJ Lipinski, N Hoehr, SJ Mazur, GL Dianov, S Senturker, M Dizdaroglu and VA Bohr
Fluorescent light (FL) has been shown to generate free radicals within
cells, however, the specific chemical nature of DNA damage induced by FL
has not previously been determined. Using gas chromatography/isotope
dilution mass spectrometry, we have detected induction of the oxidative DNA
lesions 5-hydroxycytosine (5-OH-Cyt), 2,6-diamino-4-hydroxy-5-
formamidopyrimidine (FapyGua) and 4, 6-diamino-5-formamidopyrimidine
(FapyAde) in cultured cells irradiated with FL. We followed the repair of
these lesions in normal and xeroderma pigmentosum group A (XP-A) cells.
5-OH-Cyt and FapyGua were repaired efficiently in normal cells within 6 h
following FL exposure. XP-A cells were unable to repair these oxidative DNA
base lesions. Additionally, to compare the repair of oxidative lesions
induced by various sources, in vitro repair studies were performed using
plasmid DNA damaged by FL, gamma- irradiation or OsO(4)treatment. Whole
cell extracts from normal cells repaired damaged substrates efficiently,
whereas there was little repair in XP-A extracts. Our data demon-strate
defective repair of oxidative DNA base lesions in XP-A cells in vivo and in
vitro.
ARTICLES
Repair of oxidative DNA base lesions induced by fluorescent light is defective in xeroderma pigmentosum group A cells
Laboratory of Molecular Genetics, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
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