Nucleic Acids Research, Vol 27, Issue 17 3424-3432, Copyright © 1999 by Oxford University Press
RF Hough, AT Lingam and BL Bass
The Caenorhabditis elegans T20H4.4 open reading frame (GenBank accession
no. U00037) predicted by Genefinder encodes a 367 amino acid protein that
is 32-35% identical to the C-terminal domain of adenosine deaminases that
act on RNA. We show that T20H4.4 cDNAs (GenBank accession no. AF051275)
encode a larger 495 amino acid protein that is extended at its N-terminus
to include a single double-stranded RNA- binding motif, and that T20H4.4
occupies the second position in a six- gene operon (5'-T20H4.5, T20H4.4,
R151.8A, R151.8B, R151.7, R151.6-3'). Ten different spliced-leader (SL)
sequences were found attached to T20H4.4 mRNAs, including SL1, SL2 and
eight SL2-like leaders that include two new variants. Characterization of
cDNAs derived from all six genes confirmed the essential features of
C.elegans operons: intercistronic distances in the range of 104-257 nt
between the upstream polyadenylation sites and the downstream trans -splice
sites; SL2, or SL2-like leaders, attached to the downstream mRNAs.
Polycistronic mRNA fragments revealed a 5'-untranslated region (5'-UTR)
>705 nt. The 5'-UTR is removed in mature mRNAs from the first gene
(T20H4.5) and replaced primarily by SL1, and to a lesser extent by SL2. Our
study provides new information regarding operons and how they are
processed.
ARTICLES
Caenorhabditis elegans mRNAs that encode a protein similar to ADARs derive from an operon containing six genes
Department of Biochemistry and Howard Hughes Medical Institute, University of Utah, 50 North Medical Drive, Salt Lake City, UT 84132, USA.
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