Nucleic Acids Research, Vol 27, Issue 18 3685-3689, Copyright © 1999 by Oxford University Press
TP Rasmussen, T Huang, MA Mastrangelo, J Loring, B Panning and R Jaenisch
Two protein isoforms of histone macroH2A1 (mH2A1) are found in mammalian
cells. One isoform, mH2A1.2 is highly concentrated on the
heterochromatinized inactive X chromosome (Xi) of female cells. mH2A1.2
protein is also present in male cells, but fails to form dense
concentrations. Another protein isoform, mH2A1.1, differs from mH2A1.2 by a
single short segment of amino acids. In this study, we cloned and
characterized the genomic locus of the mouse mH2A1 gene and mapped it to
chromosome 13. Two alternatively spliced transcripts derived from the mH2A1
locus are responsible for the generation of the two mH2A1 protein isoforms
with mH2A1.2 mRNA being the most abundant spliced form in all tissues
examined. The absolute amount of mH2A1 mRNA is similar in male and female
cells for most tissues with the exception of testes where it is
par-ticularly abundant. Both spliced forms are present in all adult tissues
analyzed as well as in female embryonic stem cells. In contrast, male
embryonic stem cells expressed mH2A1.1 at low levels if at all. The
relatively abundant expression of mH2A1 in both sexes suggests that mH2A1
has functions in addition to a possible involvement in X chromosome
inactivation.
ARTICLES
Messenger RNAs encoding mouse histone macroH2A1 isoforms are expressed at similar levels in male and female cells and result from alternative splicing
Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
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