Nucleic Acids Research, Vol 27, Issue 19 3851-3858, Copyright © 1999 by Oxford University Press
G Karthikeyan, KV Chary and BJ Rao
Polymerase slippage during DNA synthesis by the Klenow fragment of DNA
polymerase across A, C, G and T repeats (30 bases) has been studied. Within
minutes, duplexes that contain only repeats (30 bp) expand dramatically to
several hundred base pairs long. Rate comparisons in a repeat duplex when
one strand was expanded as against that when both strands were expanded
suggest a model of migrating hairpin loops which in the latter case
coalesce into a duplex. Moreover, slippage (at the proximal or 3'-end) is
subject to positive and negative effects from the 5'-end (distal) of the
same strand. Growing T and G strands generate T.A:T and G-G:C motif
fold-back structures at the distal end that hamper slippage at the proximal
end. On the other hand, growing tails at the distal end upon annealing with
excess complementary template accentuates proximal slippage several-fold.
ARTICLES
Fold-back structures at the distal end influence DNA slippage at the proximal end during mononucleotide repeat expansions
Department of Biological Sciences, Tata Institute of Fundamental Research, Bombay, India.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Kuwahara, S. Obika, J.-i. Nagashima, Y. Ohta, Y. Suto, H. Ozaki, H. Sawai, and T. Imanishi Systematic analysis of enzymatic DNA polymerization using oligo-DNA templates and triphosphate analogs involving 2',4'-bridged nucleosides Nucleic Acids Res., August 1, 2008; 36(13): 4257 - 4265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. D. Kelkar, S. Tyekucheva, F. Chiaromonte, and K. D. Makova The genome-wide determinants of human and chimpanzee microsatellite evolution Genome Res., January 1, 2008; 18(1): 30 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kotlyar, N. Borovok, T. Molotsky, D. Klinov, B. Dwir, and E. Kapon Synthesis of novel poly(dG)-poly(dG)-poly(dC) triplex structure by Klenow exo- fragment of DNA polymerase I Nucleic Acids Res., November 27, 2005; 33(20): 6515 - 6521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Kotlyar, N. Borovok, T. Molotsky, L. Fadeev, and M. Gozin In vitro synthesis of uniform poly(dG)-poly(dC) by Klenow exo- fragment of polymerase I Nucleic Acids Res., January 26, 2005; 33(2): 525 - 535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tasara, B. Angerer, M. Damond, H. Winter, S. Dorhofer, U. Hubscher, and M. Amacker Incorporation of reporter molecule-labeled nucleotides by DNA polymerases. II. High-density labeling of natural DNA Nucleic Acids Res., May 15, 2003; 31(10): 2636 - 2646. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Bonk, A. Humeny, J. Gebert, C. Sutter, M. v. K. Doeberitz, and C.-M. Becker Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry-based Detection of Microsatellite Instabilities in Coding DNA Sequences: A Novel Approach to Identify DNA-Mismatch Repair-deficient Cancer Cells Clin. Chem., April 1, 2003; 49(4): 552 - 561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Boyer, N. A. Yamada, C. N. Roques, S. B. Hatch, K. Riess, and R. A. Farber Sequence dependent instability of mononucleotide microsatellites in cultured mismatch repair proficient and deficient mammalian cells Hum. Mol. Genet., March 1, 2002; 11(6): 707 - 713. [Abstract] [Full Text] [PDF]
|
|