Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (471K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (23)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by van Noort, J.
Right arrow Articles by Greve, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Noort, J.
Right arrow Articles by Greve, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 27, Issue 19 3875-3880, Copyright © 1999 by Oxford University Press

ARTICLES

DNA bending by photolyase in specific and non-specific complexes studied by atomic force microscopy

J van Noort, F Orsini, A Eker, C Wyman, B de Grooth and J Greve
Applied Optics Group, Department of Applied Physics, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands.

Specific and non-specific complexes of DNA and photolyase are visualised by atomic force microscopy. As a substrate for photolyase a 1150 bp DNA restriction fragment was UV-irradiated to produce damaged sites at random positions. Comparison with a 735 bp undamaged DNA fragment made it possible to separate populations of specific and non- specific photolyase complexes on the 1150 bp fragment, relieving the need for highly defined substrates. Thus it was possible to compare DNA bending for specific and non-specific interactions. Non-specific complexes show no significant bending but increased rigidity compared to naked DNA, whereas specific complexes show DNA bending of on average 36 degrees and higher flexibility. A model obtained by docking shows that photolyase can accommodate a 36 degrees bent DNA in the vicinity of the active site.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. E. Mysiak, C. Wyman, P. E. Holthuizen, and P. C. van der Vliet
NFI and Oct-1 bend the Ad5 origin in the same direction leading to optimal DNA replication
Nucleic Acids Res., December 1, 2004; 32(21): 6218 - 6225.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Wang, Y. Yang, M. J. Schofield, C. Du, Y. Fridman, S. D. Lee, E. D. Larson, J. T. Drummond, E. Alani, P. Hsieh, et al.
DNA bending and unbending by MutS govern mismatch recognition and specificity
PNAS, December 9, 2003; 100(25): 14822 - 14827.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. van Noort, T. van der Heijden, M. de Jager, C. Wyman, R. Kanaar, and C. Dekker
The coiled-coil of the human Rad50 DNA repair protein contains specific segments of increased flexibility
PNAS, June 24, 2003; 100(13): 7581 - 7586.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Park, K. Zhang, Y. Ren, S. Nadji, N. Sinha, J.-S. Taylor, and C. Kang
Crystal structure of a DNA decamer containing a cis-syn thymine dimer
PNAS, December 10, 2002; 99(25): 15965 - 15970.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Hitomi, H. Nakamura, S.-T. Kim, T. Mizukoshi, T. Ishikawa, S. Iwai, and T. Todo
Role of Two Histidines in the (6-4) Photolyase Reaction
J. Biol. Chem., March 23, 2001; 276(13): 10103 - 10109.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.