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Nucleic Acids Research, Vol 27, Issue 2 426-428, Copyright © 1999 by Oxford University Press

ARTICLES

A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells

C Piechaczek, C Fetzer, A Baiker, J Bode and HJ Lipps
Institut fur Zellbiologie, Universitat Witten/Herdecke, Stockumer Strasse 10, D-58448 Witten, Germany.

We have developed an episomal replicating expression vector in which the SV40 gene coding for the large T-antigen was replaced by chromosomal scaffold/matrix attached regions. Southern analysis as well as vector rescue experiments in CHO cells and in Escherichia coli demonstrate that the vector replicates episomally in CHO cells. It occurs in a very low copy number in the cells and is stably maintained over more than 100 generations without selection pressure.
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