Nucleic Acids Research, Vol 27, Issue 2 462-469, Copyright © 1999 by Oxford University Press
N Reynolds, PA Fantes and SA MacNeill
Replication factor C (RF-C) is a five subunit DNA polymerase (Pol)
delta/straightepsilon accessory factor required at the replication fork for
loading the essential processivity factor PCNA onto the 3'-ends of nascent
DNA strands. Here we describe the genetic analysis of the rfc2 +gene of the
fission yeast Schizosaccharomyces pombe encoding a structural homologue of
the budding yeast Rfc2p and human hRFC37 proteins. Deletion of the rfc2 +
gene from the chromosome is lethal but does not result in the
checkpoint-dependent cell cycle arrest seen in cells deleted for the gene
encoding PCNA or for those genes encoding subunits of either Pol delta or
Pol straightepsilon. Instead, rfc2 Delta cells proceed into mitosis with
incompletely replicated DNA, indicating that the DNA replication checkpoint
is inactive under these conditions. Taken together with recent results,
these observations suggest a simple model in which assembly of the RF-C
complex onto the 3'-end of the nascent RNA-DNA primer is the last step
required for the establishment of a checkpoint-competent state.
ARTICLES
A key role for replication factor C in DNA replication checkpoint function in fission yeast
Institute of Cell and Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, UK.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Y. Yao, A. Johnson, G. D. Bowman, J. Kuriyan, and M. O'Donnell Mechanism of Proliferating Cell Nuclear Antigen Clamp Opening by Replication Factor C J. Biol. Chem., June 23, 2006; 281(25): 17528 - 17539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kobayashi, F. Figaroa, N. Meeuwenoord, L. E. T. Jansen, and G. Siegal Characterization of the DNA Binding and Structural Properties of the BRCT Region of Human Replication Factor C p140 Subunit J. Biol. Chem., February 17, 2006; 281(7): 4308 - 4317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Labib, S. E. Kearsey, and J. F.X. Diffley MCM2-7 Proteins Are Essential Components of Prereplicative Complexes that Accumulate Cooperatively in the Nucleus during G1-phase and Are Required to Establish, But Not Maintain, the S-phase Checkpoint Mol. Biol. Cell, November 1, 2001; 12(11): 3658 - 3667. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Krause, M.-L. Loupart, S. Vass, S. Schoenfelder, S. Harrison, and M. M. S. Heck Loss of Cell Cycle Checkpoint Control in Drosophila Rfc4 Mutants Mol. Cell. Biol., August 1, 2001; 21(15): 5156 - 5168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kai, H. Tanaka, and T. S.-F. Wang Fission Yeast Rad17 Associates with Chromatin in Response to Aberrant Genomic Structures Mol. Cell. Biol., May 15, 2001; 21(10): 3289 - 3301. [Abstract] [Full Text]
|
|
|
|
|
|
J. G. Moggs, P. Grandi, J.-P. Quivy, Z. O. Jónsson, U. Hübscher, P. B. Becker, and G. Almouzni A CAF-1-PCNA-Mediated Chromatin Assembly Pathway Triggered by Sensing DNA Damage Mol. Cell. Biol., February 15, 2000; 20(4): 1206 - 1218. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Kiely, S. B. Haase, P. Russell, and J. Leatherwood Functions of Fission Yeast Orp2 in DNA Replication and Checkpoint Control Genetics, February 1, 2000; 154(2): 599 - 607. [Abstract] [Full Text]
|
|
|
|
|
|
M. Shimada, D. Okuzaki, S. Tanaka, T. Tougan, K. K. Tamai, C. Shimoda, and H. Nojima Replication Factor C3 of Schizosaccharomyces pombe, a Small Subunit of Replication Factor C Complex, Plays a Role in Both Replication and Damage Checkpoints Mol. Biol. Cell, December 1, 1999; 10(12): 3991 - 4003. [Abstract] [Full Text]
|
|
|
|
|
|
S. L. G. Schmidt, A. L. Pautz, and P. M. J. Burgers ATP Utilization by Yeast Replication Factor C. IV. RFC ATP-BINDING MUTANTS SHOW DEFECTS IN DNA REPLICATION, DNA REPAIR, AND CHECKPOINT REGULATION J. Biol. Chem., September 7, 2001; 276(37): 34792 - 34800. [Abstract] [Full Text] [PDF]
|
|