Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (245K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (56)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Han, H.
Right arrow Articles by Salazar, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Han, H.
Right arrow Articles by Salazar, M.
Related Collections
Right arrow Nucleic acid structure
Right arrow Enzyme assays
Right arrow Replication
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 27, Issue 2 537-542, Copyright © 1999 by Oxford University Press

ARTICLES

A DNA polymerase stop assay for G-quadruplex-interactive compounds

H Han, LH Hurley and M Salazar
Program in Molecular Biology, Division of Medicinal Chemistry and Drug Dynamics Institute, College of Pharmacy, The University of Texas at Austin, Austin, TX 78712, USA. m-salazar@mail.utexas.edu

We have developed and characterized an assay for G-quadruplex- interactive compounds that makes use of the fact that G-rich DNA templates present obstacles to DNA synthesis by DNA polymerases. Using Taq DNA polymerase and the G-quadruplex binding 2, 6- diamidoanthraquinone BSU-1051, we find that BSU-1051 leads to enhanced arrest of DNA synthesis in the presence of K+by stabilizing an intramolecular G-quadruplex structure formed by four repeats of either TTGGGG or TTAGGG in the template strand. The data provide additional evidence that BSU-1051 modulates telomerase activity by stabilization of telomeric G-quadruplex DNA and point to a polymerase arrest assay as a sensitive method for screening for G-quadruplex-interactive agents with potential clinical utility.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
K. Guo, V. Gokhale, L. H. Hurley, and D. Sun
Intramolecularly folded G-quadruplex and i-motif structures in the proximal promoter of the vascular endothelial growth factor gene
Nucleic Acids Res., July 9, 2008; (2008) gkn380v1.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
D. Sun, W.-J. Liu, K. Guo, J. J. Rusche, S. Ebbinghaus, V. Gokhale, and L. H. Hurley
The proximal promoter region of the human vascular endothelial growth factor gene has a G-quadruplex structure that can be targeted by G-quadruplex-interactive agents
Mol. Cancer Ther., April 1, 2008; 7(4): 880 - 889.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Tang, Z.-y. Kan, Y. Yao, Q. Wang, Y.-h. Hao, and Z. Tan
G-quadruplex preferentially forms at the very 3' end of vertebrate telomeric DNA
Nucleic Acids Res., March 27, 2008; 36(4): 1200 - 1208.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. Qin, E. M. Rezler, V. Gokhale, D. Sun, and L. H. Hurley
Characterization of the G-quadruplexes in the duplex nuclease hypersensitive element of the PDGF-A promoter and modulation of PDGF-A promoter activity by TMPyP4
Nucleic Acids Res., December 3, 2007; 35(22): 7698 - 7713.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. De Cian, G. Cristofari, P. Reichenbach, E. De Lemos, D. Monchaud, M.-P. Teulade-Fichou, K. Shin-ya, L. Lacroix, J. Lingner, and J.-L. Mergny
Reevaluation of telomerase inhibition by quadruplex ligands and their mechanisms of action
PNAS, October 30, 2007; 104(44): 17347 - 17352.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. Yao, Q. Wang, Y.-h. Hao, and Z. Tan
An exonuclease I hydrolysis assay for evaluating G-quadruplex stabilization by small molecules
Nucleic Acids Res., May 14, 2007; 35(9): e68 - e68.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Cogoi and L. E. Xodo
G-quadruplex formation within the promoter of the KRAS proto-oncogene and its effect on transcription.
Nucleic Acids Res., January 1, 2006; 34(9): 2536 - 2549.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Sun, K. Guo, J. J. Rusche, and L. H. Hurley
Facilitation of a structural transition in the polypurine/polypyrimidine tract within the proximal promoter region of the human VEGF gene by the presence of potassium and G-quadruplex-interactive agents
Nucleic Acids Res., October 20, 2005; 33(18): 6070 - 6080.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M.-Y. Kim, M. Gleason-Guzman, E. Izbicka, D. Nishioka, and L. H. Hurley
The Different Biological Effects of Telomestatin and TMPyP4 Can Be Attributed to Their Selectivity for Interaction with Intramolecular or Intermolecular G-Quadruplex Structures
Cancer Res., June 15, 2003; 63(12): 3247 - 3256.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. J. Cocco, L. A. Hanakahi, M. D. Huber, and N. Maizels
Specific interactions of distamycin with G-quadruplex DNA
Nucleic Acids Res., June 1, 2003; 31(11): 2944 - 2951.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Siddiqui-Jain, C. L. Grand, D. J. Bearss, and L. H. Hurley
Direct evidence for a G-quadruplex in a promoter region and its targeting with a small molecule to repress c-MYC transcription
PNAS, September 3, 2002; 99(18): 11593 - 11598.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
W. Duan, A. Rangan, H. Vankayalapati, M.-Y. Kim, Q. Zeng, D. Sun, H. Han, O. Yu. Fedoroff, D. Nishioka, S. Y. Rha, et al.
Design and Synthesis of Fluoroquinophenoxazines That Interact with Human Telomeric G-Quadruplexes and Their Biological Effects
Mol. Cancer Ther., December 1, 2001; 1(2): 103 - 120.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Koeppel, J.-F. Riou, A. Laoui, P. Mailliet, P. B. Arimondo, D. Labit, O. Petitgenet, C. Helene, and J.-L. Mergny
Ethidium derivatives bind to G-quartets, inhibit telomerase and act as fluorescent probes for quadruplexes
Nucleic Acids Res., March 1, 2001; 29(5): 1087 - 1096.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.