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Nucleic Acids Research, Vol 27, Issue 2 649-655, Copyright © 1999 by Oxford University Press

ARTICLES

Immobilization of acrylamide-modified oligonucleotides by co- polymerization

FN Rehman, M Audeh, ES Abrams, PW Hammond, M Kenney and TC Boles
Mosaic Technologies Inc., 1106 Commonwealth Avenue, Boston, MA 02215, USA.

A flexible chemistry for solid phase attachment of oligonucleotides is described. Oligonucleotides bearing 5'-terminal acrylamide modifications efficiently co-polymerize with acrylamide monomers to form thermally stable DNA-containing polyacrylamide co-polymers. Co- polymerization attachment is specific for the terminal acrylamide group. Stable probe-containing layers are easily fabricated on supports bearing exposed acrylic groups, including plastic microtiter plates and silanized glass. Attachment can be accomplished using standard polyacrylamide gel recipes and polymerization techniques. Supports having a high surface density of hybridizable oligonucleotide (approximately 200 fmol/mm2) can be produced.
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