Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (527K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (42)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Sriskanda, V.
Right arrow Articles by Shuman, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sriskanda, V.
Right arrow Articles by Shuman, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 27, Issue 20 3953-3963, Copyright © 1999 by Oxford University Press

ARTICLES

Mutational analysis of Escherichia coli DNA ligase identifies amino acids required for nick-ligation in vitro and for in vivo complementation of the growth of yeast cells deleted for CDC9 and LIG4

V Sriskanda, B Schwer, CK Ho and S Shuman
Molecular Biology Program, Sloan-Kettering Institute, New York, NY 10021, USA.

We report that the NAD-dependent Escherichia coli DNA ligase can support the growth of Saccharomyces cerevisiae strains deleted singly for CDC9 or doubly for CDC9 plus LIG4. Alanine-scanning mutagenesis of E.coli DNA ligase led to the identification of seven amino acids (Lys115, Asp117, Asp285, Lys314, Cys408, Cys411 and Cys432) that are essential for nick-joining in vitro and for in vivo complementation in yeast. The K314A mutation uniquely resulted in accumulation of the DNA- adenylate intermediate. Alanine substitutions at five other positions (Glu113, Tyr225, Gln318, Glu319 and Cys426) did not affect in vivo complementation and had either no effect or only a modest effect on nick-joining in vitro. The E113A and Y225A mutations increased the apparent K (m)for NAD (to 45 and 76 microM, respectively) over that of the wild-type E. coli ligase (3 microM). These results are discussed in light of available structural data on the adenylylation domains of ATP- and NAD-dependent ligases. We observed that yeast cells containing only the 298-amino acid Chlorella virus DNA ligase (a 'minimal' eukaryotic ATP-dependent ligase consisting only of the catalytic core domain) are relatively proficient in the repair of DNA damage induced by UV irradiation or treatment with MMS, whereas cells containing only E.coli ligase are defective in DNA repair. This suggests that the structural domains unique to yeast Cdc9p are not essential for mitotic growth, but may facilitate DNA repair.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
L. K. Wang, H. Zhu, and S. Shuman
Structure-guided Mutational Analysis of the Nucleotidyltransferase Domain of Escherichia coli DNA Ligase (LigA)
J. Biol. Chem., March 27, 2009; 284(13): 8486 - 8494.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. K. Wang, P. A. Nair, and S. Shuman
Structure-guided Mutational Analysis of the OB, HhH, and BRCT Domains of Escherichia coli DNA Ligase
J. Biol. Chem., August 22, 2008; 283(34): 23343 - 23352.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Crut, P. A. Nair, D. A. Koster, S. Shuman, and N. H. Dekker
Dynamics of phosphodiester synthesis by DNA ligase
PNAS, May 13, 2008; 105(19): 6894 - 6899.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Akey, A. Martins, J. Aniukwu, M. S. Glickman, S. Shuman, and J. M. Berger
Crystal Structure and Nonhomologous End-joining Function of the Ligase Component of Mycobacterium DNA Ligase D
J. Biol. Chem., May 12, 2006; 281(19): 13412 - 13423.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. K. Srivastava, D. Dube, N. Tewari, N. Dwivedi, R. P. Tripathi, and R. Ramachandran
Mycobacterium tuberculosis NAD+-dependent DNA ligase is selectively inhibited by glycosylamines compared with human DNA ligase I
Nucleic Acids Res., December 15, 2005; 33(22): 7090 - 7101.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. Subramanian, S. Vijayakumar, A. E. Tomkinson, and N. Arnheim
Genetic Instability Induced by Overexpression of DNA Ligase I in Budding Yeast
Genetics, October 1, 2005; 171(2): 427 - 441.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. K. Srivastava, R. P. Tripathi, and R. Ramachandran
NAD+-dependent DNA Ligase (Rv3014c) from Mycobacterium tuberculosis: CRYSTAL STRUCTURE OF THE ADENYLATION DOMAIN AND IDENTIFICATION OF NOVEL INHIBITORS
J. Biol. Chem., August 26, 2005; 280(34): 30273 - 30281.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Zhu and S. Shuman
Structure-guided Mutational Analysis of the Nucleotidyltransferase Domain of Escherichia coli NAD+-dependent DNA Ligase (LigA)
J. Biol. Chem., April 1, 2005; 280(13): 12137 - 12144.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
M. Lavesa-Curto, H. Sayer, D. Bullard, A. MacDonald, A. Wilkinson, A. Smith, L. Bowater, A. Hemmings, and R. P. Bowater
Characterization of a temperature-sensitive DNA ligase from Escherichia coli
Microbiology, December 1, 2004; 150(12): 4171 - 4180.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Gong, A. Martins, P. Bongiorno, M. Glickman, and S. Shuman
Biochemical and Genetic Analysis of the Four DNA Ligases of Mycobacteria
J. Biol. Chem., May 14, 2004; 279(20): 20594 - 20606.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. V. Martin and S. A. MacNeill
Functional analysis of subcellular localization and protein-protein interaction sequences in the essential DNA ligase I protein of fission yeast
Nucleic Acids Res., January 29, 2004; 32(2): 632 - 642.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Georlette, V. Blaise, C. Dohmen, F. Bouillenne, B. Damien, E. Depiereux, C. Gerday, V. N. Uversky, and G. Feller
Cofactor Binding Modulates the Conformational Stabilities and Unfolding Patterns of NAD+-dependent DNA Ligases from Escherichia coli and Thermus scotoductus
J. Biol. Chem., December 12, 2003; 278(50): 49945 - 49953.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. A. Worthey, A. Schnaufer, I. S. Mian, K. Stuart, and R. Salavati
Comparative analysis of editosome proteins in trypanosomatids
Nucleic Acids Res., November 15, 2003; 31(22): 6392 - 6408.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Georlette, B. Damien, V. Blaise, E. Depiereux, V. N. Uversky, C. Gerday, and G. Feller
Structural and Functional Adaptations to Extreme Temperatures in Psychrophilic, Mesophilic, and Thermophilic DNA Ligases
J. Biol. Chem., September 26, 2003; 278(39): 37015 - 37023.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Odell, L. Malinina, V. Sriskanda, M. Teplova, and S. Shuman
Analysis of the DNA joining repertoire of Chlorella virus DNA ligase and a new crystal structure of the ligase-adenylate intermediate
Nucleic Acids Res., September 1, 2003; 31(17): 5090 - 5100.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. L. Carrick and M. D. Topal
Amino Acid Substitutions at Position 43 of NaeI Endonuclease. EVIDENCE FOR CHANGES IN NaeI STRUCTURE
J. Biol. Chem., March 7, 2003; 278(11): 9733 - 9739.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Sriskanda and S. Shuman
Role of Nucleotidyl Transferase Motif V in Strand Joining by Chlorella Virus DNA Ligase
J. Biol. Chem., March 15, 2002; 277(12): 9661 - 9667.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Sriskanda and S. Shuman
Conserved Residues in Domain Ia Are Required for the Reaction of Escherichia coli DNA Ligase with NAD+
J. Biol. Chem., March 15, 2002; 277(12): 9695 - 9700.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Sriskanda and S. Shuman
Role of nucleotidyltransferase motifs I, III and IV in the catalysis of phosphodiester bond formation by Chlorella virus DNA ligase
Nucleic Acids Res., February 15, 2002; 30(4): 903 - 911.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Sriskanda and S. Shuman
A second NAD+-dependent DNA ligase (LigB) in Escherichia coli
Nucleic Acids Res., December 15, 2001; 29(24): 4930 - 4934.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M.-A. Petit and S. D. Ehrlich
The NAD-dependent ligase encoded by yerG is an essential gene of Bacillus subtilis
Nucleic Acids Res., December 1, 2000; 28(23): 4642 - 4648.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. J. Doherty and S. W. Suh
Structural and mechanistic conservation in DNA ligases
Nucleic Acids Res., November 1, 2000; 28(21): 4051 - 4058.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Sriskanda, Z. Kelman, J. Hurwitz, and S. Shuman
Characterization of an ATP-dependent DNA ligase from the thermophilic archaeon Methanobacterium thermoautotrophicum
Nucleic Acids Res., June 1, 2000; 28(11): 2221 - 2228.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Tong, F. Barany, and W. Cao
Ligation reaction specificities of an NAD+-dependent DNA ligase from the hyperthermophile Aquifex aeolicus
Nucleic Acids Res., March 15, 2000; 28(6): 1447 - 1454.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Sriskanda, R. W. Moyer, and S. Shuman
NAD+-dependent DNA Ligase Encoded by a Eukaryotic Virus
J. Biol. Chem., September 21, 2001; 276(39): 36100 - 36109.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. V. Cherepanov and S. de Vries
Kinetic Mechanism of the Mg2+-dependent Nucleotidyl Transfer Catalyzed by T4 DNA and RNA Ligases
J. Biol. Chem., January 11, 2002; 277(3): 1695 - 1704.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.