Nucleic Acids Research, Vol 27, Issue 20 3964-3969, Copyright © 1999 by Oxford University Press
MT Hemann and CW Greider
Telomeres of eukaryotic chromosomes contain 3' overhangs which are thought
to be essential for the maintenance of proper chromosome end structure and
function. We examined the requirement for telomerase activity for the
generation of these G-strand overhangs in mammalian cells. Using
non-denaturing in-gel hybridization to both tissue and cultured cells from
mice deficient for the telomerase RNA component, we found that G-strand
overhangs exist in the absence of telomerase activity. Quantitation of
overhang signal intensity showed no significant reduction in
telomerase-deficient cells relative to wild- type. These results support a
telomerase-independent mechanism for generating G-strand overhangs.
ARTICLES
G-strand overhangs on telomeres in telomerase-deficient mouse cells
Predoctoral Training Program in Human Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
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