Nucleic Acids Research, Vol 27, Issue 20 4001-4007, Copyright © 1999 by Oxford University Press
C Dherin, JP Radicella, M Dizdaroglu and S Boiteux
We have investigated the substrate specificity of the major nuclear form of
the human Ogg1 protein, referred as alpha-hOgg1, for excision of damaged
bases from DNA exposed to gamma-irradiation. Excision products were
identified and quantified using gas chromatography/isotope dilution mass
spectrometry (GC/IDMS). The GST- alpha-hOgg1 protein used in this study is
a fusion of alpha-hOgg1 to the C-terminus of the GST protein. The results
show that GST-alpha- hOgg1 protein excises 8-hydroxyguanine (8-OH-Gua) and
2,6-diamino-4- hydroxy-5-formamidopyrimidine (FapyGua) from DNA exposed to
gamma- irradiation in a solution saturated with N(2)O or air. Fourteen
other lesions, including oxidised purines and pyrimidines, were not excised
from these substrates. Catalytic constants were measured for the excision
of 8-OH-Gua and FapyGua from DNA gamma-irradiated under N(2)O. The k (cat)/
K (m)values for excision of 8-OH-Gua and FapyGua were 4.47 x 10(-5)and 8.97
x 10(-5)(min(-1)nM(-1)), respectively. The substrate specificity and the
catalytic parameters of the wild-type GST-alpha- hOgg1 protein were
compared to that of a polymorphic form of alpha- hOgg1 harbouring a
Ser-->Cys mutation at codon 326. In the Japanese population, 47.6% of
individuals possess both alleles coding for the wild-type
alpha-hOgg1-Ser(326)and mutant alpha-hOgg1-Cys(326)proteins. The
GST-alpha-hOgg1-Cys(326)protein was purified and its substrate specificity
was determined by GC/IDMS analysis. The results show that the
GST-alpha-hOgg1-Cys(326)protein efficiently excises 8-OH-Gua and FapyGua
from gamma-irradiated DNA. The k (cat)/ K (m)values for excision of
8-OH-Gua and FapyGua were 2. 82 x 10(-5)and 4.43 x 10(- 5)(min(-1)nM(-1)),
respectively. Furthermore, we compared the capacity of these two forms of
alpha-hOgg1 to act on substrates containing 2,6- diamino-4-hydroxy-5- N
-methylformamidopyrimidine (Me-FapyGua). The k (cat)/ K (m)values for
excision of Me-FapyGua were 278 x 10(-5)and 319 x 10(-5)(min(-1)nM(-1)),
respectively. Cleavage of 34mer oligodeoxyribonucleotides containing
8-OH-Gua, 8-hydroxyadenine or an apurinic/apyrimidinic site paired with a
cytosine was also investigated. The results show that both
GST-alpha-hOgg1-Ser(326)and GST-alpha-hOgg1-Cys(326)catalyse the various
cleavage reactions at very similar rates. Furthermore, both proteins
efficiently complement the mutator phenotype of the fpg mutY mutant of
Escherichia coli.
ARTICLES
Excision of oxidatively damaged DNA bases by the human alpha-hOgg1 protein and the polymorphic alpha-hOgg1(Ser326Cys) protein which is frequently found in human populations
CEA, DSV, DRR, UMR217 CNRS-CEA, Radiobiologie Moleculaire et Cellulaire, Fontenay aux Roses, France.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. C. Stern, D. V. Conti, K. D. Siegmund, R. Corral, J.-M. Yuan, W.-P. Koh, and M. C. Yu DNA Repair Single-Nucleotide Polymorphisms in Colorectal Cancer and their Role as Modifiers of the Effect of Cigarette Smoking and Alcohol in the Singapore Chinese Health Study Cancer Epidemiol. Biomarkers Prev., November 1, 2007; 16(11): 2363 - 2372. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. A. Kuznetsov, V. V. Koval, G. A. Nevinsky, K. T. Douglas, D. O. Zharkov, and O. S. Fedorova Kinetic Conformational Analysis of Human 8-Oxoguanine-DNA Glycosylase J. Biol. Chem., January 12, 2007; 282(2): 1029 - 1038. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Paz-Elizur, R. Ben-Yosef, D. Elinger, A. Vexler, M. Krupsky, A. Berrebi, A. Shani, E. Schechtman, L. Freedman, and Z. Livneh Reduced Repair of the Oxidative 8-Oxoguanine DNA Damage and Risk of Head and Neck Cancer Cancer Res., December 15, 2006; 66(24): 11683 - 11689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kamiya, C. Cadena-Amaro, L. Dugue, H. Yakushiji, N. Minakawa, A. Matsuda, S. Pochet, Y. Nakabeppu, and H. Harashima Recognition of Nucleotide Analogs Containing the 7,8-Dihydro-8-oxo Structure by the Human MTH1 Protein J. Biochem., December 1, 2006; 140(6): 843 - 849. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. L. Nock, M. S. Cicek, L. Li, X. Liu, B. A. Rybicki, A. Moreira, S. J. Plummer, G. Casey, and J. S. Witte Polymorphisms in estrogen bioactivation, detoxification and oxidative DNA base excision repair genes and prostate cancer risk Carcinogenesis, September 1, 2006; 27(9): 1842 - 1848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Sokhansanj and D. M. Wilson III Estimating the effect of human base excision repair protein variants on the repair of oxidative DNA base damage. Cancer Epidemiol. Biomarkers Prev., May 1, 2006; 15(5): 1000 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Hill and M. K. Evans Dimerization and opposite base-dependent catalytic impairment of polymorphic S326C OGG1 glycosylase Nucleic Acids Res., March 20, 2006; 34(5): 1620 - 1632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zienolddiny, D. Campa, H. Lind, D. Ryberg, V. Skaug, L. Stangeland, D. H. Phillips, F. Canzian, and A. Haugen Polymorphisms of DNA repair genes and risk of non-small cell lung cancer Carcinogenesis, March 1, 2006; 27(3): 560 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hu, N. C. de Souza-Pinto, K. Haraguchi, B. A. Hogue, P. Jaruga, M. M. Greenberg, M. Dizdaroglu, and V. A. Bohr Repair of Formamidopyrimidines in DNA Involves Different Glycosylases: ROLE OF THE OGG1, NTH1, AND NEIL1 ENZYMES J. Biol. Chem., December 9, 2005; 280(49): 40544 - 40551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Hung, J. Hall, P. Brennan, and P. Boffetta Genetic Polymorphisms in the Base Excision Repair Pathway and Cancer Risk: A HuGE Review Am. J. Epidemiol., November 15, 2005; 162(10): 925 - 942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Kuznetsov, V. V. Koval, D. O. Zharkov, G. A. Nevinsky, K. T. Douglas, and O. S. Fedorova Kinetics of substrate recognition and cleavage by human 8-oxoguanine-DNA glycosylase Nucleic Acids Res., July 15, 2005; 33(12): 3919 - 3931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hu, S. Z. Imam, K. Hashiguchi, N. C. de Souza-Pinto, and V. A. Bohr Phosphorylation of human oxoguanine DNA glycosylase ({alpha}-OGG1) modulates its function Nucleic Acids Res., June 7, 2005; 33(10): 3271 - 3282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Hung, P. Brennan, F. Canzian, N. Szeszenia-Dabrowska, D. Zaridze, J. Lissowska, P. Rudnai, E. Fabianova, D. Mates, L. Foretova, et al. Large-Scale Investigation of Base Excision Repair Genetic Polymorphisms and Lung Cancer Risk in a Multicenter Study J Natl Cancer Inst, April 20, 2005; 97(8): 567 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Lee, N. J. Hodges, and J. K. Chipman Interindividual Variability in Response to Sodium Dichromate-Induced Oxidative DNA Damage: Role of the Ser326Cys Polymorphism in the DNA-Repair Protein of 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine DNA Glycosylase 1 Cancer Epidemiol. Biomarkers Prev., February 1, 2005; 14(2): 497 - 505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.ég. Eot-Houllier, Sév. Eon-Marchais, D. Gasparutto, and E. Sage Processing of a complex multiply damaged DNA site by human cell extracts and purified repair proteins Nucleic Acids Res., January 12, 2005; 33(1): 260 - 271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yamane, T. Kohno, K. Ito, N. Sunaga, K. Aoki, K. Yoshimura, H. Murakami, Y. Nojima, and J. Yokota Differential ability of polymorphic OGG1 proteins to suppress mutagenesis induced by 8-hydroxyguanine in human cell in vivo Carcinogenesis, September 1, 2004; 25(9): 1689 - 1694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Trzeciak, S. G. Nyaga, P. Jaruga, A. Lohani, M. Dizdaroglu, and M. K. Evans Cellular repair of oxidatively induced DNA base lesions is defective in prostate cancer cell lines, PC-3 and DU-145 Carcinogenesis, August 1, 2004; 25(8): 1359 - 1370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Katafuchi, T. Nakano, A. Masaoka, H. Terato, S. Iwai, F. Hanaoka, and H. Ide Differential Specificity of Human and Escherichia coli Endonuclease III and VIII Homologues for Oxidative Base Lesions J. Biol. Chem., April 2, 2004; 279(14): 14464 - 14471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. I. Zaika, R. A. Perlow, E. Matz, S. Broyde, R. Gilboa, A. P. Grollman, and D. O. Zharkov Substrate Discrimination by Formamidopyrimidine-DNA Glycosylase: A MUTATIONAL ANALYSIS J. Biol. Chem., February 6, 2004; 279(6): 4849 - 4861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. van der Kemp, J.-B. Charbonnier, M. Audebert, and S. Boiteux Catalytic and DNA-binding properties of the human Ogg1 DNA N-glycosylase/AP lyase: biochemical exploration of H270, Q315 and F319, three amino acids of the 8-oxoguanine-binding pocket Nucleic Acids Res., January 29, 2004; 32(2): 570 - 578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E.-Y. Cho, A. Hildesheim, C.-J. Chen, M.-M. Hsu, I-H. Chen, B. F. Mittl, P. H. Levine, M.-Y. Liu, J.-Y. Chen, L. A. Brinton, et al. Nasopharyngeal Carcinoma and Genetic Polymorphisms of DNA Repair Enzymes XRCC1 and hOGG1 Cancer Epidemiol. Biomarkers Prev., October 1, 2003; 12(10): 1100 - 1104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Gackowski, E. Speina, M. Zielinska, J. Kowalewski, R. Rozalski, A. Siomek, T. Paciorek, B. Tudek, and R. Olinski Products of Oxidative DNA Damage and Repair as Possible Biomarkers of Susceptibility to Lung Cancer Cancer Res., August 15, 2003; 63(16): 4899 - 4902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. COOKE, M. D. EVANS, M. DIZDAROGLU, and J. LUNEC Oxidative DNA damage: mechanisms, mutation, and disease FASEB J, July 1, 2003; 17(10): 1195 - 1214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Goode, C. M. Ulrich, and J. D. Potter Polymorphisms in DNA Repair Genes and Associations with Cancer Risk Cancer Epidemiol. Biomarkers Prev., December 1, 2002; 11(12): 1513 - 1530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. van Gils, R. M. Bostick, M. C. Stern, and J. A. Taylor Differences in Base Excision Repair Capacity May Modulate the Effect of Dietary Antioxidant Intake on Prostate Cancer Risk: An Example of Polymorphisms in the XRCC1 Gene Cancer Epidemiol. Biomarkers Prev., November 1, 2002; 11(11): 1279 - 1284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tuo, P. Jaruga, H. Rodriguez, M. Dizdaroglu, and V. A. Bohr The Cockayne Syndrome Group B Gene Product Is Involved in Cellular Repair of 8-Hydroxyadenine in DNA J. Biol. Chem., August 16, 2002; 277(34): 30832 - 30837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Elahi, Z. Zheng, J. Park, K. Eyring, T. McCaffrey, and P. Lazarus The human OGG1 DNA repair enzyme and its association with orolaryngeal cancer risk Carcinogenesis, July 1, 2002; 23(7): 1229 - 1234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Dantzer, L. Luna, M. Bjoras, and E. Seeberg Human OGG1 undergoes serine phosphorylation and associates with the nuclear matrix and mitotic chromatin in vivo Nucleic Acids Res., June 1, 2002; 30(11): 2349 - 2357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Sokhansanj, G. R. Rodrigue, J. P. Fitch, and D. M. W. III A quantitative model of human DNA base excision repair. I. mechanistic insights Nucleic Acids Res., April 15, 2002; 30(8): 1817 - 1825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Xu, S. L. Zheng, A. Turner, S. D. Isaacs, K. E. Wiley, G. A. Hawkins, B.-l. Chang, E. R. Bleecker, P. C. Walsh, D. A. Meyers, et al. Associations between hOGG1 Sequence Variants and Prostate Cancer Susceptibility Cancer Res., April 1, 2002; 62(8): 2253 - 2257. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. K. Hazra, T. Izumi, I. Boldogh, B. Imhoff, Y. W. Kow, P. Jaruga, M. Dizdaroglu, and S. Mitra From the Cover: Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNA PNAS, March 19, 2002; 99(6): 3523 - 3528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Mambo, S. G. Nyaga, V. A. Bohr, and M. K. Evans Defective Repair of 8-Hydroxyguanine in Mitochondria of MCF-7 and MDA-MB-468 Human Breast Cancer Cell Lines Cancer Res., March 1, 2002; 62(5): 1349 - 1355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-C. TARNG, T.-J. TSAI, W.-T. CHEN, T.-Y. LIU, and Y.-H. WEI Effect of Human OGG1 1245C->G Gene Polymorphism on 8-Hydroxy-2'-Deoxyguanosine Levels of Leukocyte DNA among Patients Undergoing Chronic Hemodialysis J. Am. Soc. Nephrol., November 1, 2001; 12(11): 2338 - 2347. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Vidal, I. D. Hickson, S. Boiteux, and J. P. Radicella Mechanism of stimulation of the DNA glycosylase activity of hOGG1 by the major human AP endonuclease: bypass of the AP lyase activity step Nucleic Acids Res., March 15, 2001; 29(6): 1285 - 1292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-H. David-Cordonnier, S. Boiteux, and P. O'Neill Excision of 8-oxoguanine within clustered damage by the yeast OGG1 protein Nucleic Acids Res., March 1, 2001; 29(5): 1107 - 1113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Shinmura, S. Yamaguchi, T. Saitoh, M. Takeuchi-Sasaki, S.-R. Kim, T. Nohmi, and J. Yokota Adenine excisional repair function of MYH protein on the adenine:8-hydroxyguanine base pair in double-stranded DNA Nucleic Acids Res., December 15, 2000; 28(24): 4912 - 4918. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Dherin, M. Dizdaroglu, H. Doerflinger, S. Boiteux, and J. P. Radicella Repair of oxidative DNA damage in Drosophila melanogaster: identification and characterization of dOgg1, a second DNA glycosylase activity for 8-hydroxyguanine and formamidopyrimidines Nucleic Acids Res., December 1, 2000; 28(23): 4583 - 4592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Audebert, S. Chevillard, C. Levalois, G. Gyapay, A. Vieillefond, J. Klijanienko, P. Vielh, A. K. El Naggar, S. Oudard, S. Boiteux, et al. Alterations of the DNA Repair Gene OGG1 in Human Clear Cell Carcinomas of the Kidney Cancer Res., September 1, 2000; 60(17): 4740 - 4744. [Abstract] [Full Text]
|
|
|
|
|
|
M. Audebert, J. P. Radicella, and M. Dizdaroglu Effect of single mutations in the OGG1 gene found in human tumors on the substrate specificity of the Ogg1 protein Nucleic Acids Res., July 15, 2000; 28(14): 2672 - 2678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nakahara, Q.-M. Zhang, K. Hashiguchi, and S. Yonei Identification of proteins of Escherichia coli and Saccharomyces cerevisiae that specifically bind to C/C mismatches in DNA Nucleic Acids Res., July 1, 2000; 28(13): 2551 - 2556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. O. Zharkov, T. A. Rosenquist, S. E. Gerchman, and A. P. Grollman Substrate Specificity and Reaction Mechanism of Murine 8-Oxoguanine-DNA Glycosylase J. Biol. Chem., September 8, 2000; 275(37): 28607 - 28617. [Abstract] [Full Text] [PDF]
|
|