Nucleic Acids Research, Vol 27, Issue 21 4167-4174, Copyright © 1999 by Oxford University Press
K Komori, N Fujita, K Ichiyanagi, H Shinagawa, K Morikawa and Y Ishino
We screened for proteins with specific binding activity to Holliday
junction DNA from the hyperthermophilic archaeon Pyrococcus furiosus and
found a protein that has specific affinity for DNA with a branched
structure, like a three-way or four-way junction. The protein was
identified as one of the two inteins encoded in the gene for ribonucleotide
reductase (RNR) by gene cloning. These two inteins were spliced out from
the precursor protein as polypeptides with molecular weights of 53.078 and
43.976 kDa, respectively. The amino acid sequences of these inteins have
two copies of the LAGLIDADG motif, which is found in the site-specific DNA
endonucleases. The purified proteins actually cleaved double-stranded DNA
with the sequence of the intein(-)allele, and, therefore, they were
designated PI- Pfu I and PI- Pfu II. They generate a 4 bp 3'-OH overhang
with a 5'-phosphate, like other known homing endonucleases originating from
inteins. The optimal conditions of the DNA cleavage reaction, including
temperature, pH, and concentrations of KCl and MgCl(2), have been
determined. The high affinity for junction DNA of PI- Pfu I was confirmed
using the purified protein.
ARTICLES
PI-PfuI and PI-PfuII, intein-coded homing endonucleases from Pyrococcus furiosus. I. Purification and identification of the homing-type endonuclease activities
Department of Molecular Biology, Biomolecular Engineering Research Institute, Suita, Osaka, Japan.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Singh, P. Tripathi, G. H. Silva, A. Pingoud, and K. Muniyappa Characterization of Mycobacterium leprae RecA Intein, a LAGLIDADG Homing Endonuclease, Reveals a Unique Mode of DNA Binding, Helical Distortion, and Cleavage Compared with a Canonical LAGLIDADG Homing Endonuclease J. Biol. Chem., September 18, 2009; 284(38): 25912 - 25928. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Watanabe, H. Yuzawa, N. Handa, and I. Kobayashi Hyperthermophilic DNA Methyltransferase M.PabI from the Archaeon Pyrococcus abyssi Appl. Envir. Microbiol., August 1, 2006; 72(8): 5367 - 5375. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ishikawa, M. Watanabe, T. Kuroita, I. Uchiyama, J. M. Bujnicki, B. Kawakami, M. Tanokura, and I. Kobayashi Discovery of a novel restriction endonuclease by genome comparison and application of a wheat-germ-based cell-free translation assay: PabI (5'-GTA/C) from the hyperthermophilic archaeon Pyrococcus abyssi Nucleic Acids Res., July 21, 2005; 33(13): e112 - e112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. H. Silva and M. Belfort Analysis of the LAGLIDADG interface of the monomeric homing endonuclease I-DmoI Nucleic Acids Res., June 9, 2004; 32(10): 3156 - 3168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-J. Noel, W. Wende, and A. Pingoud DNA Recognition by the Homing Endonuclease PI-SceI Involves a Divalent Metal Ion Cofactor-induced Conformational Change J. Biol. Chem., February 20, 2004; 279(8): 6794 - 6804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-Q. Liu, J. Yang, and Q. Meng Four Inteins and Three Group II Introns Encoded in a Bacterial Ribonucleotide Reductase Gene J. Biol. Chem., November 21, 2003; 278(47): 46826 - 46831. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Thion, E. Laurine, M. Erard, O. Burlet-Schiltz, B. Monsarrat, J.-M. Masson, and I. Saves The Two-step Cleavage Activity of PI-TfuI Intein Endonuclease Demonstrated by Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry J. Biol. Chem., November 15, 2002; 277(47): 45442 - 45450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Guhan and K. Muniyappa The RecA Intein of Mycobacterium tuberculosis Promotes Cleavage of Ectopic DNA Sites. IMPLICATIONS FOR THE DISPERSAL OF INTEINS IN NATURAL POPULATIONS J. Biol. Chem., October 18, 2002; 277(43): 40352 - 40361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Saves, C. Morlot, L. Thion, J.-L. Rolland, J. Dietrich, and J.-M. Masson Investigating the endonuclease activity of four Pyrococcus abyssi inteins Nucleic Acids Res., October 1, 2002; 30(19): 4158 - 4165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Seligman, K. M. Chisholm, B. S. Chevalier, M. S. Chadsey, S. T. Edwards, J. H. Savage, and A. L. Veillet Mutations altering the cleavage specificity of a homing endonuclease Nucleic Acids Res., September 1, 2002; 30(17): 3870 - 3879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Saves, F. Westrelin, M. Daffe, and J.-M. Masson Identification of the first eubacterial endonuclease coded by an intein allele in the pps1 gene of mycobacteria Nucleic Acids Res., November 1, 2001; 29(21): 4310 - 4318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Drouin, P. Lucas, C. Otis, C. Lemieux, and M. Turmel Biochemical characterization of I-CmoeI reveals that this H-N-H homing endonuclease shares functional similarities with H-N-H colicins Nucleic Acids Res., November 15, 2000; 28(22): 4566 - 4572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Komori, T. Miyata, J. DiRuggiero, R. Holley-Shanks, I. Hayashi, I. K. O. Cann, K. Mayanagi, H. Shinagawa, and Y. Ishino Both RadA and RadB Are Involved in Homologous Recombination in Pyrococcus furiosus J. Biol. Chem., October 20, 2000; 275(43): 33782 - 33790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Komori and Y. Ishino Replication Protein A in Pyrococcus furiosus Is Involved in Homologous DNA Recombination J. Biol. Chem., July 6, 2001; 276(28): 25654 - 25660. [Abstract] [Full Text] [PDF]
|
|