Nucleic Acids Research, Vol 27, Issue 21 4269-4275, Copyright © 1999 by Oxford University Press
BW Hargrove, A Bhattacharyya, AM Domitrovich, GM Kapler, K Kirk, DE Shippen and GR Kunkel
Telomerase is a ribonucleoprotein reverse transcriptase that synthesizes
and maintains telomeric DNA. Studies of telomeres and telomerase are
facilitated by the large number of linear DNA molecules found in ciliated
protozoa, such as Tetrahymena thermophila. To examine the expression of
telomerase, we investigated the transcription of the RNA polymerase
III-directed gene encoding the RNA subunit (TER1) of this enzyme. A
chimeric gene containing the Glaucoma chattoni TER1 transcribed region
flanked by 5' and 3' Tetrahymena regions was used to identify promoter
elements following transformation of Tetrahymena cells. Disruption of a
conserved proximal sequence element (PSE) located at -55 in the Tetrahymena
TER1 5' flanking region eliminated expression of the chimeric gene. In
addition, mutation of an A/T-rich element at -25 decreased expression
markedly. A gel mobility shift assay and protein-DNA cross-linking
identified a PSE-binding polypeptide of 50-60 kDa in Tetrahymena extracts.
Gel filtration analysis revealed a native molecular mass of approximately
160 kDa for this binding activity. Our results point to a similar
architecture between ciliate telomerase RNA and metazoan U6 small nuclear
RNA promoters.
ARTICLES
Identification of an essential proximal sequence element in the promoter of the telomerase RNA gene of Tetrahymena thermophila
Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843-2128, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Barchetta, A. La Terza, P. Ballarini, S. Pucciarelli, and C. Miceli Combination of Two Regulatory Elements in the Tetrahymena thermophila HSP70-1 Gene Controls Heat Shock Activation Eukaryot. Cell, February 1, 2008; 7(2): 379 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Skovorodkin, A. Pimenov, I. Raykhel, B. Schimanski, D. Ammermann, and A. Gunzl {alpha}-Tubulin Minichromosome Promoters in the Stichotrichous Ciliate Stylonychia lemnae Eukaryot. Cell, January 1, 2007; 6(1): 28 - 36. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. LI and E.H. BLACKBURN Expression and Suppression of Human Telomerase RNA Cold Spring Harb Symp Quant Biol, January 1, 2006; 71(0): 211 - 215. [Abstract] [PDF]
|
|
|
|
 |
|
 D. D. Cunningham and K. Collins Biological and Biochemical Functions of RNA in the Tetrahymena Telomerase Holoenzyme Mol. Cell. Biol., June 1, 2005; 25(11): 4442 - 4454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Smith, E. S. Cole, and D. P. Romero Transcriptional control of RAD51 expression in the ciliate Tetrahymena thermophila Nucleic Acids Res., August 10, 2004; 32(14): 4313 - 4321. [Abstract] [Full Text] [PDF]
|
|