Nucleic Acids Research, Vol 27, Issue 21 4291-4297, Copyright © 1999 by Oxford University Press
K Tomita, T Ueda, S Ishiwa, PF Crain, JA McCloskey and K Watanabe
Mitochondrial (mt) tRNA(Trp), tRNA(Ile), tRNA(Met), tRNA(Ser)GCU,
tRNA(Asn)and tRNA(Lys)were purified from Drosophila melanogaster (fruit
fly) and their nucleotide sequences were determined. tRNA(Lys)corresponding
to both AAA and AAG lysine codons was found to contain the anticodon CUU,
C34 at the wobble position being unmodified. tRNA(Met)corresponding to both
AUA and AUG methionine codons was found to contain 5-formylcytidine (f(5)C)
at the wobble position, although the extent of modification is partial.
These results suggest that both C and f(5)C as the wobble bases at the
anticodon first position (position 34) can recognize A at the codon third
position (position 3) in the fruit fly mt translation system. tRNA(Ser)GCU
corresponding to AGU, AGC and AGA serine codons was found to contain
unmodified G at the anticodon wobble position, suggesting the utilization
of an unconventional G34-A3 base pair during translation. When these tRNA
anticodon sequences are compared with those of other animal counterparts,
it is concluded that either unmodified C or G at the wobble position can
recognize A at the codon third position and that modification from A to
t(6)A at position 37, 3'-adjacent to the anticodon, seems to be important
for tRNA possessing C34 to recognize A3 in the mRNA in the fruit fly mt
translation system.
ARTICLES
Codon reading patterns in Drosophila melanogaster mitochondria based on their tRNA sequences: a unique wobble rule in animal mitochondria
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan.
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