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Nucleic Acids Research, Vol 27, Issue 22 4363-4368, Copyright © 1999 by Oxford University Press

ARTICLES

Localisation of a reporter transcript by the c-myc 3'-UTR is linked to translation

GD Dalgleish, JL Veyrune, N Accornero, JM Blanchard and JE Hesketh
Intracellular Targeting Group, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, UK.

The 3'-untranslated region of c-myc mRNA contains a perinuclear localisation signal which is sufficient to target beta-globin coding sequences. The link between perinuclear mRNA localisation and translation has been investigated using cells transfected with chimeric gene constructs in which globin reporter sequences were linked to the c- myc 3'-untranslated region and the iron-responsive element from ferritin mRNA. Iron supplementation of the medium promoted translation of the chimeric mRNA as assessed by its presence in polysomes; in situ hybridisation showed that the mRNA was localised around the nucleus. Treatment with the iron chelator desferrioxamine for 16 h prevented both translation and mRNA localisation. In controls where the expressed mRNA lacked the iron-responsive element desferrioxamine had no effect upon localisation. In contrast, arrest of on-going global translation by puromycin treatment had no effect on mRNA localisation. The data suggest that if initiation of translation of a mRNA containing the c- myc localisation signal is prevented in some way then localisation does not occur, whereas once the mRNA has been localised further translation is not required to maintain mRNA localisation.
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