Nucleic Acids Research, Vol 27, Issue 22 4385-4390, Copyright © 1999 by Oxford University Press
N Murata-Kamiya, H Kamiya, N Karino, Y Ueno, H Kaji, A Matsuda and H Kasai
5-methyl-2'-deoxycytidine (5-Me-dC) is formed by the enzymatic methylation
of dC, primarily in CpG sequences in DNA, and is involved in the regulation
of gene expression. In the present study, 5-Me-dC and double-stranded DNA
fragments containing 5-Me-dC were either gamma- irradiated or aerobically
treated with Fenton-type reagents, Fe(II)- EDTA, Fe(II)-nitrilotriacetic
acid, Fe(III)-EDTA-H(2)O(2)-catechol or ascorbic acid-H(2)O(2) under
neutral conditions. The formation of 5- formyl-2'-deoxycytidine (5-CHO-dC)
was observed upon treatment of both 5-Me-dC and DNA fragments containing
5-Me-dC. The yields of 5-CHO-dC from 5-Me-dC and those of
5-formyl-2'-deoxyuridine from dT were comparable. These results suggest
that 5-Me-dC in DNA is as susceptible to oxidation as dT in cells, and
raise the possibility that 5-CHO-dC may contribute to the high mutagenic
rate observed in CpG sequences in genomic DNA.
ARTICLES
Formation of 5-formyl-2'-deoxycytidine from 5-methyl-2'-deoxycytidine in duplex DNA by Fenton-type reactions and gamma-irradiation
Department of Health Policy and Management, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan.
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