Nucleic Acids Research, Vol 27, Issue 22 4468-4475, Copyright © 1999 by Oxford University Press
W Lin, H Xin, Y Zhang, X Wu, F Yuan and Z Wang
DNA is frequently damaged by various physical and chemical agents. DNA
damage can lead to mutations during replication. In the yeast Saccharomyces
cerevisiae, the damage-induced mutagenesis pathway requires the Rev1
protein. We have isolated a human cDNA homologous to the yeast REV1 gene.
The human REV1 cDNA consists of 4255 bp and codes for a protein of 1251
amino acid residues with a calculated molecular weight of 138 248 Da. The
human REV1 gene is localized between 2q11.1 and 2q11.2. We show that the
human REV1 protein is a dCMP transferase that specifically inserts a dCMP
residue opposite a DNA template G. In addition, the human REV1 transferase
is able to efficiently and specifically insert a dCMP opposite a DNA
template apurinic/apyrimidinic (AP) site or a uracil residue. These results
suggest that the REV1 transferase may play a critical role during mutagenic
translesion DNA synthesis bypassing a template AP site in human cells.
Consistent with its role as a fundamental mutagenic protein, the REV1 gene
is ubiquitously expressed in various human tissues.
ARTICLES
The human REV1 gene codes for a DNA template-dependent dCMP transferase
Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40536, USA.
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C.W. LAWRENCE, P.E.M. GIBBS, R.S. MURANTE, X.-D. WANG, Z. LI, T.P. MCMANUS, W.G. MCGREGOR, J.R. NELSON, D.C. HINKLE, and V.M. MAHER Roles of DNA Polymerase {zeta} and Rev1 Protein in Eukaryotic Mutagenesis and Translesion Replication Cold Spring Harb Symp Quant Biol, January 1, 2000; 65(0): 61 - 70. [Abstract] [PDF]
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Y. Masuda, M. Takahashi, S. Fukuda, M. Sumii, and K. Kamiya Mechanisms of dCMP Transferase Reactions Catalyzed by Mouse Rev1 Protein J. Biol. Chem., January 18, 2002; 277(4): 3040 - 3046. [Abstract] [Full Text] [PDF]
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Z. Livneh DNA Damage Control by Novel DNA Polymerases: Translesion Replication and Mutagenesis J. Biol. Chem., July 6, 2001; 276(28): 25639 - 25642. [Full Text] [PDF]
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C. L. Limoli, E. Giedzinski, W. F. Morgan, and J. E. Cleaver Inaugural Article: Polymerase eta deficiency in the xeroderma pigmentosum variant uncovers an overlap between the S phase checkpoint and double-strand break repair PNAS, July 5, 2000; 97(14): 7939 - 7946. [Abstract] [Full Text] [PDF]
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