Nucleic Acids Research, Vol 27, Issue 24 4830-4837, Copyright © 1999 by Oxford University Press
SO Kelley, EM Boon, JK Barton, NM Jackson and MG Hill
High-throughput DNA sensors capable of detecting single-base mismatches are
required for the routine screening of genetic mutations and disease. A new
strategy for the electrochemical detection of single- base mismatches in
DNA has been developed based upon charge transport through DNA films.
Double-helical DNA films on gold surfaces have been prepared and used to
detect DNA mismatches electrochemically. The signals obtained from
redox-active intercalators bound to DNA-modified gold surfaces display a
marked sensitivity to the presence of base mismatches within the
immobilized duplexes. Differential mismatch detection was accomplished
irrespective of DNA sequence composition and mismatch identity. Single-base
changes in sequences hybridized at the electrode surface are also detected
accurately. Coupling the redox reactions of intercalated species to
electrocatalytic processes in solution considerably increases the
sensitivity of this assay. Reporting on the electronic structure of DNA, as
opposed to the hybridization energetics of single-stranded
oligonucleotides, electrochemical sensors based on charge transport may
offer fundamental advantages in both scope and sensitivity.
ARTICLES
Single-base mismatch detection based on charge transduction through DNA
Division of Chemistry, California Institute of Technology, Pasadena, CA 91125, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. A. Gorodetsky, L. E. P. Dietrich, P. E. Lee, B. Demple, D. K. Newman, and J. K. Barton DNA binding shifts the redox potential of the transcription factor SoxR PNAS, March 11, 2008; 105(10): 3684 - 3689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. DeRosa, A. Sancar, and J. K. Barton Supramolecular Structure and Dynamics Special Feature: Electrically monitoring DNA repair by photolyase PNAS, August 2, 2005; 102(31): 10788 - 10792. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Shen, M. F. G. Anand, and R. Levicky X-ray photoelectron spectroscopy and infrared spectroscopy study of maleimide-activated supports for immobilization of oligodeoxyribonucleotides Nucleic Acids Res., November 10, 2004; 32(20): 5973 - 5980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Koehne, H. Chen, A. M. Cassell, Q. Ye, J. Han, M. Meyyappan, and J. Li Miniaturized Multiplex Label-Free Electronic Chip for Rapid Nucleic Acid Analysis Based on Carbon Nanotube Nanoelectrode Arrays Clin. Chem., October 1, 2004; 50(10): 1886 - 1893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Xie, Y. H. Yu, P.-L. Mao, and Z. Gao Highly sensitive amperometric detection of genomic DNA in animal tissues Nucleic Acids Res., January 20, 2004; 32(2): e15 - e15. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Meunier-Prest, S. Raveau, E. Finot, G. Legay, M. Cherkaoui-Malki, and N. Latruffe Direct measurement of the melting temperature of supported DNA by electrochemical method Nucleic Acids Res., December 1, 2003; 31(23): e150 - e150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Boon, A. L. Livingston, N. H. Chmiel, S. S. David, and J. K. Barton DNA-mediated charge transport for DNA repair PNAS, October 28, 2003; 100(22): 12543 - 12547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Mao, C. Luo, and Q. Ouyang Studies of temperature-dependent electronic transduction on DNA hairpin loop sensor Nucleic Acids Res., September 15, 2003; 31(18): e108 - e108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Fan, K. W. Plaxco, and A. J. Heeger Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA PNAS, August 5, 2003; 100(16): 9134 - 9137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. K. Bhattacharya, J. Cha, and J. K. Barton 1H NMR determination of base-pair lifetimes in oligonucleotides containing single base mismatches Nucleic Acids Res., November 1, 2002; 30(21): 4740 - 4750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Song, F. Zhou, J. Wang, N. Tao, J. Lin, R. L. Vellanoweth, Y. Morquecho, and J. Wheeler-Laidman Detection of oligonucleotide hybridization at femtomolar level and sequence-specific gene analysis of the Arabidopsis thaliana leaf extract with an ultrasensitive surface plasmon resonance spectrometer Nucleic Acids Res., July 15, 2002; 30(14): e72 - e72. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-J. Park, T. A. Taton, and C. A. Mirkin Array-Based Electrical Detection of DNA with Nanoparticle Probes Science, February 22, 2002; 295(5559): 1503 - 1506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Boone and G. B. Schuster Long-range oxidative damage in duplex DNA: the effect of bulged G in a G-C tract and tandem G/A mispairs Nucleic Acids Res., February 1, 2002; 30(3): 830 - 837. [Abstract] [Full Text] [PDF]
|
|