Nucleic Acids Research, Vol 27, Issue 24 e37-e37, Copyright © 1999 by Oxford University Press
MA Marra, TA Kucaba, LW Hillier and RH Waterston
To accommodate the increasingly rapid rates of DNA sequencing we have
developed and implemented an inexpensive, expeditious method for the
purification of double-stranded plasmid DNA clones. The robust nature, high
throughput, low degree of technical difficulty and extremely low cost have
made it the plasmid DNA preparation method of choice in both our expressed
sequence tag (EST) and genome sequencingprojects. Here we report the
details of the method and describe its application in the generation of
more than 700 000 ESTs at a rate exceeding 16 000 per week.
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High-throughput plasmid DNA purification for 3 cents per sample
Washington University Genome Sequencing Center, 4444 Forest Park Boulevard, St Louis, MI 63108, USA
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