Nucleic Acids Research, Vol 27, Issue 3 831-838, Copyright © 1999 by Oxford University Press
D Yean and JD Gralla
Potential pathways that could account for observed rapid rates of
transcription reinitiation were explored. A nuclear extract system was
established in which reinitiation rates were observed to be kinetically
facilitated and in which the rate was sensitive to TATA box mutation.
Kinetic facilitation of functional complex formation could be mimicked by
pre-assembling activator and certain general transcription factors on the
promoter and then adding nuclear extract. The minimal activated complex
with this characteristic contained general factors TFIID and TFIIA. The
ability of the TFIID:TFIIA complex to complete assembly rapidly was reduced
by the same TATA box mutation that reduced reinitiation rate. Band shift
experiments also showed that this same mutation lowered the stability of
the TBP:TFIIA complex on the DNA. The results suggest that TATA-dependent
variations in retention of the TFIID:TFIIA complex after release of the
polymerase could be a primary determinant of reinitiation rate, allowing
diversity in promoter strength to be related to diversity in TATA element
sequences.
ARTICLES
Transcription reinitiation rate: a potential role for TATA box stabilization of the TFIID:TFIIA:DNA complex
Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095- 1569, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. T. Knuesel, K. D. Meyer, C. Bernecky, and D. J. Taatjes The human CDK8 subcomplex is a molecular switch that controls Mediator coactivator function Genes & Dev., February 15, 2009; 23(4): 439 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. F. Murphy, G. Balazsi, and J. J. Collins Combinatorial promoter design for engineering noisy gene expression PNAS, July 31, 2007; 104(31): 12726 - 12731. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. C. Lin and J. D. Gralla Stimulation of the XPB ATP-dependent helicase by the beta subunit of TFIIE Nucleic Acids Res., May 25, 2005; 33(9): 3072 - 3081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Upadhyaya, M. Khan, T.-C. Mou, M. Junker, D. M. Gray, and J. DeJong The Germ Cell-specific Transcription Factor ALF. STRUCTURAL PROPERTIES AND STABILIZATION OF THE TATA-BINDING PROTEIN (TBP)-DNA COMPLEX J. Biol. Chem., September 6, 2002; 277(37): 34208 - 34216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-a Kim, J. Lu, and P. G. Quinn Distinct cAMP response element-binding protein (CREB) domains stimulate different steps in a concerted mechanism of transcription activation PNAS, October 10, 2000; 97(21): 11292 - 11296. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. S. Wolner and J. D. Gralla Roles for Non-TATA Core Promoter Sequences in Transcription and Factor Binding Mol. Cell. Biol., May 15, 2000; 20(10): 3608 - 3615. [Abstract] [Full Text]
|
|
|
|
|
|
K.-T. Jeang, H. Xiao, and E. A. Rich Multifaceted Activities of the HIV-1 Transactivator of Transcription, Tat J. Biol. Chem., October 8, 1999; 274(41): 28837 - 28840. [Full Text] [PDF]
|
|
|
|
|
|
B. S. Wolner and J. D. Gralla TATA-flanking Sequences Influence the Rate and Stability of TATA-binding Protein and TFIIB Binding J. Biol. Chem., February 23, 2001; 276(9): 6260 - 6266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Woodard, K.-j. Lee, J. Huang, and W. S. Dynan Distinct Roles for Ku Protein in Transcriptional Reinitiation and DNA Repair J. Biol. Chem., April 27, 2001; 276(18): 15423 - 15433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Stewart and L. A. Stargell The Stability of the TFIIA-TBP-DNA Complex Is Dependent on the Sequence of the TATAAA Element J. Biol. Chem., August 3, 2001; 276(32): 30078 - 30084. [Abstract] [Full Text] [PDF]
|
|