Nucleic Acids Research, Vol 27, Issue 3 854-858, Copyright © 1999 by Oxford University Press
J Sloan, JR Kinghorn and SE Unkles
Molybdoenzymes are ubiquitous and require a prosthetic group called the
molybdenum cofactor for activity. We provide evidence here that the two
heteromeric subunits (MOCO1-A and MOCO1-B) of human molybdopterin synthase,
which is involved in the conversion of precursor Z to molybdopterin in the
molybdenum cofactor biosynthetic pathway, are spe- cified by a single
bicistronic mRNA with overlapping reading frames. The transcript is in low
abundance and shows variable tissue distribution. We propose that leaky
scanning of the first translational initiation codon for MOCO1-A by 40S
ribosomal subunits occurs, allowing recognition of the AUG for the
downstream MOCO1-B reading frame. Such a genetic arrangement may result in
a constant ratio and close proximity of lowly expressed enzyme subunits
which should, a priori, be especially advantageous for assembly in complex
mammalian cells. The MOCO1 locus resides on human chromosome 5.
ARTICLES
The two subunits of human molybdopterin synthase: evidence for a bicistronic messenger RNA with overlapping reading frames
Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia andSchool of Environmental and Evolutionary Biology, University of St Andrews, St Andrews, Fife KY16 9TH, UK.
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