Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (34K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (55)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kenzelmann, M.
Right arrow Articles by Muhlemann, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kenzelmann, M.
Right arrow Articles by Muhlemann, K.
Related Collections
Right arrow Cloning
Right arrow Miscellaneous/other
Right arrow Monitoring gene expression
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 27, Issue 3 917-918, Copyright © 1999 by Oxford University Press

ARTICLES

Substantially enhanced cloning efficiency of SAGE (Serial Analysis of Gene Expression) by adding a heating step to the original protocol

M Kenzelmann and K Muhlemann
Institute of Medical Microbiology, University of Bern, Friedbuhlstrasse 51, 3010 Bern, Switzerland.

The efficiency of the original SAGE (Serial Analysis of Gene Expression) protocol was limited by a small average size of cloned concatemers. We describe a modification of the technique that overcomes this problem. Ligation of ditags yields concatemers of various sizes. Small concatemers may aggregate and migrate with large ones during gel electrophoresis. A heating step introduced before gel electrophoresis breaks such contaminating aggregates. This modification yields cloned concatemers with an average size of 67 tags as compared to 22 tags by the original protocol. It enhances the length of cloned concatemers substantially and reduces the costs of SAGE.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 IOVSHome page
C. B. Lupien, C. Bolduc, S. Landreville, and C. Salesse
Comparison between the Gene Expression Profile of Human Muller Cells and Two Spontaneous Muller Cell Lines
Invest. Ophthalmol. Vis. Sci., November 1, 2007; 48(11): 5229 - 5242.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
M. N. Ashby, J. Rine, E. F. Mongodin, K. E. Nelson, and D. Dimster-Denk
Serial Analysis of rRNA Genes and the Unexpected Dominance of Rare Members of Microbial Communities
Appl. Envir. Microbiol., July 15, 2007; 73(14): 4532 - 4542.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
L. J. Hathaway, S. Brugger, A. Martynova, S. Aebi, and K. Muhlemann
Use of the Agilent 2100 Bioanalyzer for Rapid and Reproducible Molecular Typing of Streptococcus pneumoniae
J. Clin. Microbiol., March 1, 2007; 45(3): 803 - 809.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
J. Khattra, A. D. Delaney, Y. Zhao, A. Siddiqui, J. Asano, H. McDonald, P. Pandoh, N. Dhalla, A.-l. Prabhu, K. Ma, et al.
Large-scale production of SAGE libraries from microdissected tissues, flow-sorted cells, and cell lines
Genome Res., January 1, 2007; 17(1): 108 - 116.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
W.-M. Boon, T. Beissbarth, L. Hyde, G. Smyth, J. Gunnersen, D. A. Denton, H. Scott, and S.-S. Tan
A comparative analysis of transcribed genes in the mouse hypothalamus and neocortex reveals chromosomal clustering
PNAS, October 12, 2004; 101(41): 14972 - 14977.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
C Bolduc, M Larose, M Yoshioka, P Ye, P Belleau, C Labrie, J Morissette, V Raymond, F Labrie, and J St-Amand
Effects of dihydrotestosterone on adipose tissue measured by serial analysis of gene expression
J. Mol. Endocrinol., October 1, 2004; 33(2): 429 - 444.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. P. So, R. F. B. Turner, and C. A. Haynes
Increasing the efficiency of SAGE adaptor ligation bydirected ligation chemistry
Nucleic Acids Res., July 6, 2004; 32(12): e96 - e96.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Gowda, C. Jantasuriyarat, R. A. Dean, and G.-L. Wang
Robust-LongSAGE (RL-SAGE): A Substantially Improved LongSAGE Method for Gene Discovery and Transcriptome Analysis
Plant Physiology, March 1, 2004; 134(3): 890 - 897.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
F. Schwartz, A. Duka, E. Triantafyllidi, C. Johns, I. Duka, J. Cui, and H. Gavras
Serial analysis of gene expression in mouse kidney following angiotensin II administration
Physiol Genomics, December 16, 2003; 16(1): 90 - 98.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. YOSHIOKA, H. TANAKA, N. SHONO, E. E. SNYDER, M. SHINDO, and J. ST-AMAND
Serial analysis of gene expression in the skeletal muscle of endurance athletes compared to sedentary men
FASEB J, October 1, 2003; 17(13): 1812 - 1819.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
I. Schwering, A. Brauninger, U. Klein, B. Jungnickel, M. Tinguely, V. Diehl, M.-L. Hansmann, R. Dalla-Favera, K. Rajewsky, and R. Kuppers
Loss of the B-lineage-specific gene expression program in Hodgkin and Reed-Sternberg cells of Hodgkin lymphoma
Blood, February 15, 2003; 101(4): 1505 - 1512.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
J. J. Dunn, S. R. McCorkle, L. A. Praissman, G. Hind, D. van der Lelie, W. F. Bahou, D. V. Gnatenko, and M. K. Krause
Genomic Signature Tags (GSTs): A System for Profiling Genomic DNA
Genome Res., November 1, 2002; 12(11): 1756 - 1765.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
O. Potapova, S. V. Anisimov, M. Gorospe, R. H. Dougherty, W. A. Gaarde, K. R. Boheler, and N. J. Holbrook
Targets of c-Jun NH2-terminal Kinase 2-mediated Tumor Growth Regulation Revealed by Serial Analysis of Gene Expression
Cancer Res., June 1, 2002; 62(11): 3257 - 3263.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
A. J. Kreeft, C. J.A. Moen, M. H. Hofker, R. R. Frants, E. Vreugdenhil, M. J.J. Gijbels, L. M. Havekes, and N. A. Datson
Identification of Differentially Regulated Genes in Mildly Hyperlipidemic ApoE3-Leiden Mice by Use of Serial Analysis of Gene Expression
Arterioscler. Thromb. Vasc. Biol., December 1, 2001; 21(12): 1984 - 1990.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. H. Margulies, S. L. R. Kardia, and J. W. Innis
Identification and prevention of a GC content bias in SAGE libraries
Nucleic Acids Res., June 15, 2001; 29(12): e60 - e60.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
K. Polyak and G. J. Riggins
Gene Discovery Using the Serial Analysis of Gene Expression Technique: Implications for Cancer Research
J. Clin. Oncol., June 1, 2001; 19(11): 2948 - 2958.
[Abstract] [Full Text] [PDF]

Home page
 Eur J Heart FailHome page
S. V. Anisimov, E. G. Lakatta, and K. R. Boheler
Discovering altered genomic expression patterns in heart: transcriptome determination by serial analysis of gene expression
Eur J Heart Fail, June 1, 2001; 3(3): 271 - 281.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. Waghray, M. Schober, F. Feroze, F. Yao, J. Virgin, and Y. Q. Chen
Identification of Differentially Expressed Genes by Serial Analysis of Gene Expression in Human Prostate Cancer
Cancer Res., May 1, 2001; 61(10): 4283 - 4286.
[Abstract] [Full Text]

Home page
 Nucleic Acids ResHome page
M. J. Hayden and P. J. Sharp
Sequence-tagged microsatellite profiling (STMP): a rapid technique for developing SSR markers
Nucleic Acids Res., April 15, 2001; 29(8): e43 - e43.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J. ST-AMAND, K. OKAMURA, K. MATSUMOTO, S. SHIMIZU, and Y. SOGAWA
Characterization of control and immobilized skeletal muscle: an overview from genetic engineering
FASEB J, March 1, 2001; 15(3): 684 - 692.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. M. Angelastro, L. P. Klimaschewski, and O. V. Vitolo
Improved NlaIII digestion of PAGE-purified 102 bp ditags by addition of a single purification step in both the SAGE and microSAGE protocols
Nucleic Acids Res., June 15, 2000; 28(12): e62 - e62.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.