Nucleic Acids Research, Vol 27, Issue 5 1283-1288, Copyright © 1999 by Oxford University Press
NI Zanchin and DS Goldfarb
The Saccharomyces cerevisiae protein Rrp43p co-purifies with four other
3'-->5' exoribonucleases in a complex that has been termed the exosome.
Rrp43p itself is similar to prokaryotic RNase PH. Individual exosome
subunits have been implicated in the 3' maturation of the 5.8S rRNA found
in 60S ribosomes and the 3' degradation of mRNAs. However, instead of being
deficient in 60S ribosomes, Rrp43p-depleted cells were deficient in 40S
ribosomes. Pulse-chase and steady-state northern analyses of pre-RNA and
rRNA levels revealed a significant delay in the synthesis of both 25S and
18S rRNAs, accompanied by the stable accumulation of 35S and 27S pre-rRNAs
and the under-accumulation of 20S pre-rRNA. In addition, Rrp43p-depleted
cells accumulated a 23S aberrant pre-rRNA and a fragment excised from the
5' ETS. Therefore, in addition to the maturation of 5.8S rRNA, Rrp43p is
required for the maturation 18S and 25S rRNA.
ARTICLES
The exosome subunit Rrp43p is required for the efficient maturation of 5.8S, 18S and 25S rRNA
Department of Biology, University of Rochester, Rochester, NY 14627, USA.
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