Nucleic Acids Research, Vol 27, Issue 5 1338-1344, Copyright © 1999 by Oxford University Press
MF Dubois, NF Marshall, VT Nguyen, GK Dahmus, F Bonnet, ME Dahmus and O Bensaude
Reversible phosphorylation of the C-terminal domain (CTD) of the largest
RNA polymerase II (RNAP II) subunit plays a key role in gene expression.
Stresses such as heat shock result in marked changes in CTD phosphorylation
as well as in major alterations in gene expression. CTD kinases and CTD
phosphatase(s) contribute in mediating differential CTD phosphory-lation.
We now report that heat shock of HeLa cells at temperatures as mild as 41
degreesC results in a decrease in CTD phosphatase activity in cell
extracts. The obser-vation that this CTD phosphatase interacts with the
RAP74 subunit of the general transcription factor TFIIF suggests that it
corresponds to the previously charac-terized major CTD phosphatase. This
conclusion is also supported by the finding that the distribution of the
150 kDa subunit of CTD phosphatase in cells is altered by heat shock.
Although CTD phosphatase is found predominantly in low salt extracts in
unstressed cells, immunofluorescence microscopy indicates that its
intracellular localization is nuclear. The decrease in CTD phosphatase
activity correlates with a decrease in amount of 150 kDa phosphatase
subunit in the extracts. During heat shock, CTD phosphatase switches to an
insoluble form which remains aggregated to the nuclear matrix fraction. In
contrast, heat shock did not result in a redistribution of RAP74,
indicating that not all nuclear proteins aggregate under these conditions.
Accordingly, the heat-inactivation of both the CTD phosphatase and the
TFIIH-associated CTD kinase might contribute to the selective synthesis of
heat-shock mRNAs.
ARTICLES
Heat shock of HeLa cells inactivates a nuclear protein phosphatase specific for dephosphorylation of the C-terminal domain of RNA polymerase II
Laboratoire de Genetique Moleculaire, Ecole Normale Superieure, 46 rue d'Ulm, 75230 Paris Cedex 05, France.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. K. Rose and C. H. Rankin Blocking memory reconsolidation reverses memory-associated changes in glutamate receptor expression. J. Neurosci., November 8, 2006; 26(45): 11582 - 11587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Boehm, A. Saunders, J. Werner, and J. T. Lis Transcription Factor and Polymerase Recruitment, Modification, and Movement on dhsp70 In Vivo in the Minutes following Heat Shock Mol. Cell. Biol., November 1, 2003; 23(21): 7628 - 7637. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Palancade, M.-F. Dubois, and O. Bensaude FCP1 Phosphorylation by Casein Kinase 2 Enhances Binding to TFIIF and RNA Polymerase II Carboxyl-terminal Domain Phosphatase Activity J. Biol. Chem., September 20, 2002; 277(39): 36061 - 36067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.-J. Cho, M. S. Kobor, M. Kim, J. Greenblatt, and S. Buratowski Opposing effects of Ctk1 kinase and Fcp1 phosphatase at Ser 2 of the RNA polymerase II C-terminal domain Genes & Dev., December 15, 2001; 15(24): 3319 - 3329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mathew, S. K. Mathur, C. Jolly, S. G. Fox, S. Kim, and R. I. Morimoto Stress-Specific Activation and Repression of Heat Shock Factors 1 and 2 Mol. Cell. Biol., November 1, 2001; 21(21): 7163 - 7171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Palancade, M. F. Dubois, M. E. Dahmus, and O. Bensaude Transcription-Independent RNA Polymerase II Dephosphorylation by the FCP1 Carboxy-Terminal Domain Phosphatase in Xenopus laevis Early Embryos Mol. Cell. Biol., October 1, 2001; 21(19): 6359 - 6368. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Licciardo, L. Ruggiero, L. Lania, and B. Majello Transcription activation by targeted recruitment of the RNA polymerase II CTD phosphatase FCP1 Nucleic Acids Res., September 1, 2001; 29(17): 3539 - 3545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Casse, F. Giannoni, V. T. Nguyen, M.-F. Dubois, and O. Bensaude The Transcriptional Inhibitors, Actinomycin D and alpha -Amanitin, Activate the HIV-1 Promoter and Favor Phosphorylation of the RNA Polymerase II C-terminal Domain J. Biol. Chem., June 4, 1999; 274(23): 16097 - 16106. [Abstract] [Full Text] [PDF]
|
|