Nucleic Acids Research, Vol 27, Issue 5 1350-1358, Copyright © 1999 by Oxford University Press
E Frolova, M Johnston and J Majors
Binding of the MIG1 repressor to the glucose-repressible GAL1 and GAL4
promoters was analyzed in vivo by cyclobutane dimer footprinting in two
yeast strains that show different glucose repression responses. Mig1p
binding to the two promoters in both strains was glucose-induced. In cells
subject to rapid and stringent glucose repression (S288c), long- term Mig1p
binding in glucose-grown cells was inhibited by the formation of a
competing chromatin structure. Under conditions where glucose repression
was only partially effective (gal80 - or low glucose), the chromatin
structure did not form and long-term Mig1p binding was observed The same
long-term binding of Mig1p was seen in cells of a different strain (W303A)
that shows only partial glucose repression of the GAL1 promoter. We
conclude from these experiments that Mig1p binding to glucose-repressed
promoters is glucose-dependent but transient. We suggest that Mig1p
functions at an early step in repression, but is not required to maintain
the repressed state.
ARTICLES
Binding of the glucose-dependent Mig1p repressor to the GAL1 and GAL4 promoters in vivo: regulationby glucose and chromatin structure
Department of Biochemistry and Molecular Biophysics and Department of Genetics, Washington UniversitySchool of Medicine, 660 South Euclid, St Louis, MO 63110, USA.
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