Nucleic Acids Research, Vol 27, Issue 5 1377-1385, Copyright © 1999 by Oxford University Press
KT Militello and LK Read
Mitochondrial RNAs in Trypanosoma brucei are post-transcriptionally
modified by the addition and deletion of uridylate residues in a process
called kRNA editing. Unedited, partially edited and fully edited RNAs exist
in the steady-state RNA population. Previous experiments have demonstrated
that T.brucei mitochondrial RNAs contain both short (approximately 20 nt)
and long (120-200 nt) poly(A) tracts. However, it is unknown exactly what
poly(A) tract lengths are present on unedited, partially edited and fully
edited RNAs. To gain insight into the role of the poly(A) tract in T.brucei
mitochondria, ribosomal protein S12 (RPS12) RNAs with short and long
poly(A) tracts were purified by hybrid selection and analyzed by RT-PCR and
DNA sequencing. Unedited RPS12 RNAs were found almost exclusively in
populations with short poly(A) tracts. Both partially and fully edited
RPS12 RNAs were found in populations with short and long poly(A) tracts.
Therefore, there is a correlation between the presence of editing and the
presence of the long poly(A) tract. Since a proportion of partially edited
RPS12 RNAs contain long poly(A) tracts, it is unlikely that the long
poly(A) tract is the sole signal for translation. Other implications for
the role of polyadenylation in mitochondrial gene regulation are discussed.
ARTICLES
Coordination of kRNA editing and polyadenylation in Trypanosoma brucei mitochondria: complete editing is not required for long poly(A) tract addition
Department of Microbiology and Center for Microbial Pathogenesis, SUNY at Buffalo School of Medicine, Buffalo, NY 14214, USA.
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