Nucleic Acids Research, Vol 27, Issue 8 1828-1836, Copyright © 1999 by Oxford University Press
BJ Thiele, M Berger, A Huth, I Reimann, K Schwarz and H Thiele
By screening a rabbit reticulocyte library, an alternative 15-LOX
transcript of 3.6 kb (15-LOX mRNA2) was detected containing a 1019 nt
longer 3'-untranslated region (UTR2) than the main 2.6 kb mRNA (15-LOX
mRNA1). In anaemic animals, northern blotting showed that 15-LOX mRNA2 was
predominantly expressed in non-erythroid tissues, whereas 15-LOX mRNA1 was
exclusively expressed in red blood cells and bone marrow. The 15-LOX
3'-UTR2 mRNA2 contained a novel 8-fold repetitive CU-rich motif, 23 nt in
length (DICE2). This motif is related but not identical to the 10-fold
repetitive differentiation control element (DICE1) of 19 nt residing in the
15-LOX UTR1 mRNA1. DICE1 was shown to interact with human hnRNP proteins E1
and K, thereby inhibiting translation. From tissues expressing the long
15-LOX mRNA2, two to three unidentified polypeptides with molecular weights
of 53-55 and 90-93 kDa which bound to DICE2 were isolated by RNA affinity
chromatography. A 93 kDa protein from lung cytosol, which was selected by
DICE2 binding, was able to suppress translational inhibition of 15-LOX
mRNA2, but not of 15-LOX mRNA1, by hnRNP E1. A possible interaction between
DICE1/DICE2 cis / trans factors in translational control of 15-LOX
synthesis is discussed. Furthermore, the 3'-terminal part of the highly
related rabbit leukocyte-type 12-LOX gene was analysed. Very similar
repetitive CU-rich elements of the type DICE1 (20 repeats) and DICE2 (nine
repeats) were found in the part corresponding to the 3'-UTR of the mRNA.
ARTICLES
Tissue-specific translational regulation of alternative rabbit 15- lipoxygenase mRNAs differing in their 3'-untranslated regions
Institute of Biochemistry, University Clinics Charite, Humboldt- University Berlin, Hessische Strasse 3-4, D-10115 Berlin, Germany.
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