Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (790K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (169)
Right arrowRequest Permissions
Citing Articles
Right arrowScopus Links
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Beier, M.
Right arrow Articles by Hoheisel, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Beier, M.
Right arrow Articles by Hoheisel, J. D.
Related Collections
Right arrow Genomics
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 27, Issue 9 1970-1977, Copyright © 1999 by Oxford University Press

ARTICLES

Versatile derivatisation of solid support media for covalent bonding on DNA-microchips

M Beier and JD Hoheisel
Functional Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 506, D-69120 Heidelberg, Germany. m.beier@dkfz- heidelberg.de

A chemistry was developed that permits on DNA-arrays both the covalent immobilisation of pre-fabricated nucleic acids-such as oligonucleotides, PCR-products or peptide nucleic acid oligomers-and the in situ synthesis of such compounds on either glass or polypropylene surfaces. Bonding was found to be stable even after some 30 cycles of stripping. Due to a dendrimeric structure of the linker molecule, the loading can be modified in a controlled manner and increased beyond the capacity of glass without negative effects on hybridisation efficiency. Also, the chemistry warrants the modulation of other surface properties such as charge or hydrophobicity. Preferentially, attachment of nucleic acids takes place only via the terminal amino-group of amino-modified oligonucleotides or the terminal hydroxyl-group of unmodified molecules so that the entire molecule is accessible to probe hybridisation. This derivatisation represents a support chemistry versatile enough to serve nearly all current forms of DNA-arrays or microchips.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. F. Phillips, M. R. Lockett, M. J. Rodesch, M. R. Shortreed, F. Cerrina, and L. M. Smith
In situ oligonucleotide synthesis on carbon materials: stable substrates for microarray fabrication
Nucleic Acids Res., January 17, 2008; 36(1): e7 - e7.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. R. Sabanayagam and J. R. Lakowicz
Increasing the sensitivity of DNA microarrays by metal-enhanced fluorescence using surface-bound silver nanoparticles
Nucleic Acids Res., January 28, 2007; 35(2): e13 - e13.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Kinoshita, K. Fujimoto, T. Yakabe, S. Saito, Y. Hamaguchi, T. Kikuchi, K. Nonaka, S. Murata, D. Masuda, W. Takada, et al.
Multiple primer extension by DNA polymerase on a novel plastic DNA array coated with a biocompatible polymer
Nucleic Acids Res., January 12, 2007; 35(1): e3 - e3.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. Zhang, T. Hurek, and B. Reinhold-Hurek
Position of the fluorescent label is a crucial factor determining signal intensity in microarray hybridizations
Nucleic Acids Res., October 27, 2005; 33(19): e166 - e166.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Liu and G. C. Bazan
Methods for strand-specific DNA detection with cationic conjugated polymers suitable for incorporation into DNA chips and microarrays
PNAS, January 18, 2005; 102(3): 589 - 593.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Fixe, V. Chu, D. M. F. Prazeres, and J. P. Conde
An on-chip thin film photodetector for the quantification of DNA probes and targets in microarrays
Nucleic Acids Res., May 17, 2004; 32(9): e70 - e70.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Kimura, R. Oda, Y. Inaki, and O. Suzuki
Attachment of oligonucleotide probes to poly carbodiimide-coated glass for microarray applications
Nucleic Acids Res., April 23, 2004; 32(7): e68 - e68.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. S. Seo, X. Bai, H. Ruparel, Z. Li, N. J. Turro, and J. Ju
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
PNAS, April 13, 2004; 101(15): 5488 - 5493.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Fixe, M. Dufva, P. Telleman, and C. B. V. Christensen
Functionalization of poly(methyl methacrylate) (PMMA) as a substrate for DNA microarrays
Nucleic Acids Res., January 12, 2004; 32(1): e9 - e9.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Santulli-Marotto, S. K. Nair, C. Rusconi, B. Sullenger, and E. Gilboa
Multivalent RNA Aptamers That Inhibit CTLA-4 and Enhance Tumor Immunity
Cancer Res., November 1, 2003; 63(21): 7483 - 7489.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Le Berre, E. Trevisiol, A. Dagkessamanskaia, S. Sokol, A.-M. Caminade, J. P. Majoral, B. Meunier, and J. Francois
Dendrimeric coating of glass slides for sensitive DNA microarrays analysis
Nucleic Acids Res., August 15, 2003; 31(16): e88 - e88.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Lagorce, N. C. Hauser, D. Labourdette, C. Rodriguez, H. Martin-Yken, J. Arroyo, J. D. Hoheisel, and J. Francois
Genome-wide Analysis of the Response to Cell Wall Mutations in the Yeast Saccharomyces cerevisiae
J. Biol. Chem., May 23, 2003; 278(22): 20345 - 20357.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
J. Peplies, F. O. Glockner, and R. Amann
Optimization Strategies for DNA Microarray-Based Detection of Bacteria with 16S rRNA-Targeting Oligonucleotide Probes
Appl. Envir. Microbiol., March 1, 2003; 69(3): 1397 - 1407.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Raddatz, J. Mueller-Ibeler, J. Kluge, L. Wass, G. Burdinski, J. R. Havens, T. J. Onofrey, D. Wang, and M. Schweitzer
Hydrazide oligonucleotides: new chemical modification for chip array attachment and conjugation
Nucleic Acids Res., November 1, 2002; 30(21): 4793 - 4802.
[Abstract] [Full Text] [PDF]

Home page
 J Gerontol A Biol Sci Med SciHome page
E. Wang, C. Lacelle, S. Xu, X. Zhao, and M. Hou
Designer Microarrays: From Soup To Nuts
J Gerontol A Biol Sci Med Sci, November 1, 2002; 57(11): B400 - B405.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
V. Chizhikov, M. Wagner, A. Ivshina, Y. Hoshino, A. Z. Kapikian, and K. Chumakov
Detection and Genotyping of Human Group A Rotaviruses by Oligonucleotide Microarray Hybridization
J. Clin. Microbiol., July 1, 2002; 40(7): 2398 - 2407.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. Bordoni, C. Consolandi, B. Castiglioni, E. Busti, L. R. Bernardi, C. Battaglia, and G. De Bellis
Investigation of the multiple anchors approach in oligonucleotide microarray preparation using linear and stem-loop structured probes
Nucleic Acids Res., April 15, 2002; 30(8): e34 - e34.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. A. Podyminogin, E. A. Lukhtanov, and M. W. Reed
Attachment of benzaldehyde-modified oligodeoxynucleotide probes to semicarbazide-coated glass
Nucleic Acids Res., December 15, 2001; 29(24): 5090 - 5098.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. L. Dolan, Y. Wu, L. K. Ista, R. L. Metzenberg, M. A. Nelson, and G. P. Lopez
Robust and efficient synthetic method for forming DNA microarrays
Nucleic Acids Res., November 1, 2001; 29(21): e107 - e107.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Lindroos, U. Liljedahl, M. Raitio, and A.-C. Syvanen
Minisequencing on oligonucleotide microarrays: comparison of immobilisation chemistries
Nucleic Acids Res., July 1, 2001; 29(13): e69 - e69.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. LeProust, H. Zhang, P. Yu, X. Zhou, and X. Gao
Characterization of oligodeoxyribonucleotide synthesis on glass plates
Nucleic Acids Res., May 15, 2001; 29(10): 2171 - 2180.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Kumar and Z. Liang
Chemical nanoprinting: a novel method for fabricating DNA microchips
Nucleic Acids Res., January 15, 2001; 29(2): e2 - e2.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Adessi, G. Matton, G. Ayala, G. Turcatti, J.-J. Mermod, P. Mayer, and E. Kawashima
Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms
Nucleic Acids Res., October 15, 2000; 28(20): e87 - e87.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Kumar, O. Larsson, D. Parodi, and Z. Liang
Silanized nucleic acids: a general platform for DNA immobilization
Nucleic Acids Res., July 15, 2000; 28(14): e71 - e71.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Afanassiev, V. Hanemann, and S. Wolfl
Preparation of DNA and protein micro arrays on glass slides coated with an agarose film
Nucleic Acids Res., June 15, 2000; 28(12): e66 - e66.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Beier and J. D. Hoheisel
Production by quantitative photolithographic synthesis of individually quality checked DNA microarrays
Nucleic Acids Res., February 15, 2000; 28(4): e11 - e11.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.