Nucleic Acids Research, Vol 27, Issue 9 2006-2014, Copyright © 1999 by Oxford University Press
M Homann and HU Goringer
African trypanosomiasis is a parasitic disease caused by a specific class
of protozoan organisms. The best-studied representative of that group is
Trypanosoma brucei which is transmitted by tsetse flies and multiplies in
the blood of many mammals. Trypanosomes evade the immune system by altering
their surface structure which is dominated by a layer of a variant surface
glycoprotein (VSG). Although invariant surface proteins exist, they are
inaccessible to the humoral immune response. Using a combinatorial
selection method in conjunction with live trypanosomes as the binding
target, we show that short RNA ligands (aptamers) for constant surface
components can be isolated. We describe the selection of three classes of
RNA aptamers that crosslink to a single 42 kDa protein located within the
flagellar pocket of the parasite. The RNAs associate rapidly and with high
affinity. They do not discriminate between two different trypanosome VSG
variant strains and, furthermore, are able to bind to other trypanosome
strains not used in the selection protocol. Thus, the aptamers have the
potential to function as markers on the surface of the extracellular
parasite and as such they might be modified to function as novel drugs
against African trypanosomiasis.
ARTICLES
Combinatorial selection of high affinity RNA ligands to live African trypanosomes
Laboratorium fur Molekulare Biologie, Genzentrum der Ludwig-Maximilians- Universitat Munchen, Am Klopferspitz 18a, 82152 Martinsried, Germany.
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