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Nucleic Acids Research, 2000, Vol. 28, No. 1 81-84
© 2000 Oxford University Press

TRIPLES: a database of gene function in Saccharomyces cerevisiae

Anuj Kumar, Kei-Hoi Cheung1, Petra Ross-Macdonald, Paulo S. R. Coelho, Perry Miller1 and Michael Snyder*

Department of Molecular, Cellular and Developmental Biology, Yale University, PO Box 208103, New Haven, CT 06520-8103, USA and 1Center for Medical Informatics, Yale University School of Medicine, 333 Cedar Street, PO Box 208009, New Haven, CT 06520-8009, USA

Using a novel multipurpose mini-transposon, we have generated a collection of defined mutant alleles for the analysis of disruption phenotypes, protein localization, and gene expression in Saccharomyces cerevisiae. To catalog this unique data set, we have developed TRIPLES, a Web-accessible database of TRansposon-Insertion Phenotypes, Localization and Expression in Saccharomyces. Encompassing over 250 000 data points, TRIPLES provides convenient access to information from nearly 7800 transposon-mutagenized yeast strains; within TRIPLES, complete data reports of each strain may be viewed in table format, or if desired, downloaded as tab-delimited text files. Each report contains external links to corresponding entries within the Saccharomyces Genome Database and International Nucleic Acid Sequence Data Library (GenBank). Unlike other yeast databases, TRIPLES also provides on-line order forms linked to each clone report; users may immediately request any desired strain free-of-charge by submitting a completed form. In addition to presenting a wealth of information for over 2300 open reading frames, TRIPLES constitutes an important medium for the distribution of useful reagents throughout the yeast scientific community. Maintained by the Yale Genome Analysis Center, TRIPLES may be accessed at http://ycmi.med.yale.edu/ygac/triples.htm

* To whom correspondence should be addressed. Tel: +1 203 432 6139; Fax: +1 203 432 6161; Email: michael.snyder@yale.edu


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