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Nucleic Acids Research, 2000, Vol. 28, No. 1 94-96
© 2000 Oxford University Press

Gene discovery using the maize genome database ZmDB

Xiaowu Gai, Shailesh Lal, Liqun Xing, Volker Brendel* and Virginia Walbot1

Department of Zoology and Genetics, Iowa State University, Ames, IA 50011-3260, USA and 1Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020, USA

Zea mays DataBase (ZmDB) is a repository and analysis tool for sequence, expression and phenotype data of the major crop plant maize. The data accessible in ZmDB are mostly generated in a large collaborative project of maize gene discovery, sequencing and phenotypic analysis using a transposon tagging strategy and expressed sequence tag (EST) sequencing. ESTs constitute most of the current content. Database search tools, convenient links to external databases, and novel sequence analysis programs for spliced alignment are provided and together serve as an efficient protocol for gene discovery by sequence inspection. ZmDB can be accessed at http://zmdb. iastate.edu . ZmDB also provides web-based ordering of materials generated in the project, including EST and genomic DNA clones, seeds of mutant plants and microarrays of amplified EST and genomic DNA sequences.

* To whom correspondence should be addressed. Tel: +1 515 294 9884; Fax: +1 515 294 6755; Email: vbrendel@iastate.edu


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