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Nucleic Acids Research, 2000, Vol. 28, No. 10 2060-2068
© 2000 Oxford University Press

DNA repair in a yeast origin of replication: contributions of photolyase and nucleotide excision repair

Bernhard Suter, Ralf-Erik Wellinger and Fritz Thoma*

Institut für Zellbiologie, ETH-Zürich, Hönggerberg, CH-8093 Zürich, Switzerland

DNA damage formation and repair are tightly linked to protein–DNA interactions in chromatin. We have used minichromosomes in yeast as chromatin substrates in vivo to investigate how nucleotide excision repair (NER) and repair by DNA-photolyase (photoreactivation) remove pyrimidine dimers from an origin of replication (ARS1). The ARS1 region is nuclease sensitive and flanked by nucleosomes on both sides. Photoreactivation was generally faster than NER at all sites. Site-specific heterogeneity of repair was observed for both pathways. This heterogeneity was different for NER and photoreactivation and it was altered in a minichromosome where ARS1 was transcribed. The results indicate distinct inter­actions of the repair systems with protein complexes bound in the ARS region (ORC, Abf1) and a predomi­nant role of photolyase in CPD repair of an origin of replication.

* To whom correspondence should be addressed. Tel: +41 1 6333323; Fax: +41 1 6331069; Email: thoma@cell.biol.ethz.ch


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