Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (287K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (62)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Yu, F.
Right arrow Articles by Strätling, W. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, F.
Right arrow Articles by Strätling, W. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 10 2201-2206
© 2000 Oxford University Press

Histone deacetylase-independent transcriptional repression by methyl-CpG-binding protein 2

Fang Yu, Jens Thiesen and Wolf H. Strätling*

Institut für Medizinische Biochemie und Molekularbiologie, Universitäts-Krankenhaus Eppendorf, Martinistraße 52, D-20246 Hamburg, Germany

Methyl-CpG-binding protein 2 (MeCP2) contains a transcriptional repression domain (TRD), which can act by recruitment of a large transcriptional co-repressor complex containing histone deacetylases HDAC1 and 2. We demonstrate here that transient transcription from the SV40 enhancer/promoter or the SV40 promoter is strongly repressed in a histone deacetylase-independent manner, since repression is not alleviated by Trichostatin A (TSA). In a mutational analysis, repression depends on a conserved 30 residue sequence containing two clusters of basic amino acids. Mutation of the first of these clusters inhibits in vitro interaction between TRD and mSin3A. Furthermore, a subdomain of the TRD containing the conserved 30-residue sequence and 16 flanking amino acids was sufficient to compromise VP16-activated transcription. In summary, our results indicate an alternative, histone deacetylase-independent pathway of transcriptional repression by MeCP2.

* To whom correspondence should be addressed. Tel: +49 40 42803 2392; Fax: +49 40 42803 4862; Email: straetli@uke.uni-hamburg.de


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Cancer ResHome page
F. Wischnewski, O. Friese, K. Pantel, and H. Schwarzenbach
Methyl-CpG Binding Domain Proteins and Their Involvement in the Regulation of the MAGE-A1, MAGE-A2, MAGE-A3, and MAGE-A12 Gene Promoters
Mol. Cancer Res., July 1, 2007; 5(7): 749 - 759.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Nikitina, X. Shi, R. P. Ghosh, R. A. Horowitz-Scherer, J. C. Hansen, and C. L. Woodcock
Multiple Modes of Interaction between the Methylated DNA Binding Protein MeCP2 and Chromatin
Mol. Cell. Biol., February 1, 2007; 27(3): 864 - 877.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. C. Galvao and J. O. Thomas
Structure-specific binding of MeCP2 to four-way junction DNA through its methyl CpG-binding domain
Nucleic Acids Res., November 27, 2005; 33(20): 6603 - 6609.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
A K E Swales and N Spears
Genomic imprinting and reproduction
Reproduction, October 1, 2005; 130(4): 389 - 399.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. M. Springer and S. M. Kaeppler
Evolutionary Divergence of Monocot and Dicot Methyl-CpG-Binding Domain Proteins
Plant Physiology, May 1, 2005; 138(1): 92 - 104.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-G. Jin, C.-L. Jiang, T. Rauch, H. Li, and G. P. Pfeifer
MBD3L2 Interacts with MBD3 and Components of the NuRD Complex and Can Oppose MBD2-MeCP1-mediated Methylation Silencing
J. Biol. Chem., April 1, 2005; 280(13): 12700 - 12709.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
K. N. Thatcher, S. Peddada, D. H. Yasui, and J. M. LaSalle
Homologous pairing of 15q11-13 imprinted domains in brain is developmentally regulated but deficient in Rett and autism samples
Hum. Mol. Genet., March 15, 2005; 14(6): 785 - 797.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
W. Zhao, H. Soejima, K. Higashimoto, T. Nakagawachi, T. Urano, S. Kudo, S. Matsukura, S. Matsuo, K. Joh, and T. Mukai
The Essential Role of Histone H3 Lys9 Di-Methylation and MeCP2 Binding in MGMT Silencing with Poor DNA Methylation of the Promoter CpG Island
J. Biochem., March 1, 2005; 137(3): 431 - 440.
[Abstract] [Full Text] [PDF]

Home page
 J. Med. Genet.Home page
L S Weaving, C J Ellaway, J Gecz, and J Christodoulou
Rett syndrome: clinical review and genetic update
J. Med. Genet., January 1, 2005; 42(1): 1 - 7.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C.-L. Jiang, S.-G. Jin, and G. P. Pfeifer
MBD3L1 Is a Transcriptional Repressor That Interacts with Methyl-CpG-binding Protein 2 (MBD2) and Components of the NuRD Complex
J. Biol. Chem., December 10, 2004; 279(50): 52456 - 52464.
[Abstract] [Full Text] [PDF]

Home page
 NeuroscientistHome page
P. R. Albert and S. Lemonde
5-HT1A Receptors, Gene Repression, and Depression: Guilt by Association
Neuroscientist, December 1, 2004; 10(6): 575 - 593.
[Abstract] [PDF]

Home page
 Hum Mol GenetHome page
A. L. Collins, J. M. Levenson, A. P. Vilaythong, R. Richman, D. L. Armstrong, J. L. Noebels, J. David Sweatt, and H. Y. Zoghbi
Mild overexpression of MeCP2 causes a progressive neurological disorder in mice
Hum. Mol. Genet., November 1, 2004; 13(21): 2679 - 2689.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Fujita, S. Watanabe, T. Ichimura, Y. Ohkuma, T. Chiba, H. Saya, and M. Nakao
MCAF Mediates MBD1-Dependent Transcriptional Repression
Mol. Cell. Biol., April 15, 2003; 23(8): 2834 - 2843.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Koizume, K. Tachibana, T. Sekiya, S. Hirohashi, and M. Shiraishi
Heterogeneity in the modification and involvement of chromatin components of the CpG island of the silenced human CDH1 gene in cancer cells
Nucleic Acids Res., November 1, 2002; 30(21): 4770 - 4780.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A.-Y. Xie and W. R. Folk
Inhibition of Polyomavirus ori-Dependent DNA Replication by mSin3B
J. Virol., October 25, 2002; 76(23): 11809 - 11818.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Detich, J. Theberge, and M. Szyf
Promoter-specific Activation and Demethylation by MBD2/Demethylase
J. Biol. Chem., September 20, 2002; 277(39): 35791 - 35794.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Roder, M.-J. Latasa, and H. S. Sul
Silencing of the Mouse H-rev107 Gene Encoding a Class II Tumor Suppressor by CpG Methylation
J. Biol. Chem., August 16, 2002; 277(34): 30543 - 30550.
[Abstract] [Full Text] [PDF]

Home page
 Cell Growth Differ.Home page
J. M. Ordway and T. Curran
Methylation Matters: Modeling a Manageable Genome
Cell Growth Differ., April 1, 2002; 13(4): 149 - 162.
[Full Text] [PDF]

Home page
 J. Immunol.Home page
G. P. White, P. M. Watt, B. J. Holt, and P. G. Holt
Differential Patterns of Methylation of the IFN-{gamma} Promoter at CpG and Non-CpG Sites Underlie Differences in IFN-{gamma} Gene Expression Between Human Neonatal and Adult CD45RO- T Cells
J. Immunol., March 15, 2002; 168(6): 2820 - 2827.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. A. Drewell, C. J. Goddard, J. O. Thomas, and M. A. Surani
Methylation-dependent silencing at the H19 imprinting control region by MeCP2
Nucleic Acids Res., March 1, 2002; 30(5): 1139 - 1144.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. C. Lorincz, D. Schubeler, and M. Groudine
Methylation-Mediated Proviral Silencing Is Associated with MeCP2 Recruitment and Localized Histone H3 Deacetylation
Mol. Cell. Biol., December 1, 2001; 21(23): 7913 - 7922.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Yu, N. Zingler, G. Schumann, and W. H. Stratling
Methyl-CpG-binding protein 2 represses LINE-1 expression and retrotransposition but not Alu transcription
Nucleic Acids Res., November 1, 2001; 29(21): 4493 - 4501.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Benjamin and J.-P. Jost
Reversal of methylation-mediated repression with short-chain fatty acids: evidence for an additional mechanism to histone deacetylation
Nucleic Acids Res., September 1, 2001; 29(17): 3603 - 3610.
[Abstract] [Full Text] [PDF]

Home page
 J. Gerontol. A Biol. Sci. Med. Sci.Home page
R. Yung, D. Ray, J. K. Eisenbraun, C. Deng, J. Attwood, M. D. Eisenbraun, K. Johnson, R. A. Miller, S. Hanash, and B. Richardson
Unexpected Effects of a Heterozygous Dnmt1 Null Mutation on Age-Dependent DNA Hypomethylation and Autoimmunity
J. Gerontol. A Biol. Sci. Med. Sci., June 1, 2001; 56(6): B268 - B276.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. Wan, K. Zhao, S. S. J. Lee, and U. Francke
MECP2 truncating mutations cause histone H4 hyperacetylation in Rett syndrome
Hum. Mol. Genet., May 1, 2001; 10(10): 1085 - 1092.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. M. Yusufzai and A. P. Wolffe
Functional consequences of Rett syndrome mutations on human MeCP2
Nucleic Acids Res., November 1, 2000; 28(21): 4172 - 4179.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. Dragich, I. Houwink-Manville, and C. Schanen
Rett syndrome: a surprising result of mutation in MECP2
Hum. Mol. Genet., October 1, 2000; 9(16): 2365 - 2375.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Roder, M.-S. Hung, T.-L. Lee, T.-Y. Lin, H. Xiao, K.-I. Isobe, J.-L. Juang, and C.-K. J. Shen
Transcriptional Repression by Drosophila Methyl-CpG-Binding Proteins
Mol. Cell. Biol., October 1, 2000; 20(19): 7401 - 7409.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
J. Boeke, O. Ammerpohl, S. Kegel, U. Moehren, and R. Renkawitz
The Minimal Repression Domain of MBD2b Overlaps with the Methyl-CpG-binding Domain and Binds Directly to Sin3A
J. Biol. Chem., November 3, 2000; 275(45): 34963 - 34967.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Free, R. I. D. Wakefield, B. O. Smith, D. T. F. Dryden, P. N. Barlow, and A. P. Bird
DNA Recognition by the Methyl-CpG Binding Domain of MeCP2
J. Biol. Chem., January 26, 2001; 276(5): 3353 - 3360.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Rodda, S. Sharma, M. Scherer, G. Chapman, and P. Rathjen
CRTR-1, a Developmentally Regulated Transcriptional Repressor Related to the CP2 Family of Transcription Factors
J. Biol. Chem., January 26, 2001; 276(5): 3324 - 3332.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.