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Nucleic Acids Research, 2000, Vol. 28, No. 11 E56-e56
© 2000 Oxford University Press

BtrI, a novel restriction endonuclease, recognises the non-palindromic sequence 5'-CACGTC(-3/-3)-3'

Sergei Kh. Degtyarev, Olga A. Belichenko, Natalya A. Lebedeva, Vladimir S. Dedkov and Murat A. Abdurashitov*

SibEnzyme, 9 Ak.Timakov Str., Novosibirsk 630117, Russia

The recognition sequence and cleavage positions of a new restriction endonuclease BtrI isolated from Bacillus stearothermophilus SE-U62 have been determined. BtrI belongs to a rare type IIQ of restriction endonucleases, which recognise non-palindromic nucleotide sequences and cleave DNA symmetrically within them.

* To whom correspondence should be addressed. Tel: +7 3832 335499; Fax +7 3832 336853; Email: se_info@ma.nsc.ru


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