Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (849K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (23)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Bagga, R.
Right arrow Articles by Emerson, B. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bagga, R.
Right arrow Articles by Emerson, B. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 13 2541-2550
© 2000 Oxford University Press

HMG I/Y regulates long-range enhancer-dependent transcription on DNA and chromatin by changes in DNA topology

Rajesh Bagga, Susan Michalowski1, Rohit Sabnis, Jack D. Griffith1 and Beverly M. Emerson*

Regulatory Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA and 1Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA

The nature of nuclear structures that are required to confer transcriptional regulation by distal enhancers is unknown. We show that long-range enhancer-dependent ß-globin transcription is achieved in vitro upon addition of the DNA architectural protein HMG I/Y to affinity-enriched holo RNA polymerase II complexes. In this system, HMG I/Y represses promoter activity in the absence of an associated enhancer and strongly activates transcription in the presence of a distal enhancer. Importantly, nucleosome formation is neither necessary for long-range enhancer regulation in vitro nor sufficient without the addition of HMG I/Y. Thus, the modulation of DNA structure by HMG I/Y is a critical regulator of long-range enhancer function on both DNA and chromatin-assembled genes. Electron microscopic analysis reveals that HMG I/Y binds cooperatively to preferred DNA sites to generate distinct looped structures in the presence or absence of the ß-globin enhancer. The formation of DNA topologies that enable distal enhancers to strongly regulate gene expression is an intrinsic property of HMG I/Y and naked DNA.

* To whom correspondence should be addressed. Tel: +1 858 453 6560; Fax: +1 858 535 8194; Email: emerson@salk.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Cancer ResHome page
I. Cleynen, J. R. Brants, K. Peeters, R. Deckers, M. Debiec-Rychter, R. Sciot, W. J.M. Van de Ven, and M. M.R. Petit
HMGA2 Regulates Transcription of the Imp2 Gene via an Intronic Regulatory Element in Cooperation with Nuclear Factor-{kappa}B
Mol. Cancer Res., April 1, 2007; 5(4): 363 - 372.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Florquin, Y. Saeys, S. Degroeve, P. Rouze, and Y. Van de Peer
Large-scale structural analysis of the core promoter in mammalian and plant genomes
Nucleic Acids Res., July 27, 2005; 33(13): 4255 - 4264.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Rombauts, K. Florquin, M. Lescot, K. Marchal, P. Rouze, and Y. Van de Peer
Computational Approaches to Identify Promoters and cis-Regulatory Elements in Plant Genomes
Plant Physiology, July 1, 2003; 132(3): 1162 - 1176.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
I. Bouallaga, S. Teissier, M. Yaniv, and F. Thierry
HMG-I(Y) and the CBP/p300 Coactivator Are Essential for Human Papillomavirus Type 18 Enhanceosome Transcriptional Activity
Mol. Cell. Biol., April 1, 2003; 23(7): 2329 - 2340.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Subramanian and J. D. Griffith
Interactions between p53, hMSH2-hMSH6 and HMG I(Y) on Holliday junctions and bulged bases
Nucleic Acids Res., June 1, 2002; 30(11): 2427 - 2434.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. G. Sargent and J. A. Lloyd
The Human gamma -Globin TATA and CACCC Elements Have Key, Distinct Roles in Suppressing beta -Globin Gene Expression in Embryonic/Fetal Development
J. Biol. Chem., November 2, 2001; 276(45): 41817 - 41824.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
M. F. Shannon, L. S. Coles, J. Attema, and P. Diamond
The role of architectural transcription factors in cytokine gene transcription
J. Leukoc. Biol., January 1, 2001; 69(1): 21 - 32.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.