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Nucleic Acids Research, 2000, Vol. 28, No. 13 E70-e70
© 2000 Oxford University Press

New specificity and yield enhancer of polymerase chain reactions

Martina Kovárová and Petr Dráber*

Department of Mammalian Gene Expression, Institute of Molecular Genetics, Academy of Science of the Czech Republic, Vídenská 1083, 142 20 Prague 4, Czech Republic

Tetramethylammonium (TMA) chloride, dimethyl sulfoxide and formamide are known to increase, under certain conditions, the specificity and efficiency of the polymerase chain reaction (PCR). We compared the ability of several TMA derivatives and some other reagents to increase the specificity of PCR and to improve the yield of amplification. A novel combination of the enhancer TMA and oxalate as anion is demonstrated to be a powerful enhancer of PCR. Addition of 2 mM TMA oxalate to the PCR mixture decreases the formation of non-specific DNA fragments and increases the yield of specific PCR products.

* To whom correspondence should be addressed. Tel: +420 2 475 2468; Fax: +420 2 4147 0339; Email: draberpe@biomed.cas.cz


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