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Nucleic Acids Research, 2000, Vol. 28, No. 14 2784-2793
© 2000 Oxford University Press

In Rhizobium leguminosarum, NodD represses its own transcription by competing with RNA polymerase for binding sites

Hailiang Hu, Songtao Liu, Yang Yang, Weizhong Chang and Guofan Hong*

Shanghai Institute of Biochemistry, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China

We isolated RNA polymerase (RNAP) from Rhizobium leguminosarum, the nitrogen-fixing symbiont of peas and Vicia. Its 91 kDa subunit, which is homologous to {sigma}70 of Escherichia coli RNAP, is necessary for transcription of the regulatory nodD gene, which in the presence of certain flavonoids induces transcription of other nod genes that are needed for the early steps of infection. We also show that negative autoregulation of nodD was achieved through competition between RNAP and NodD for their respective binding sites, which largely overlap. Combined with the result that high concentrations of the flavonoid inducer naringenin relieved the binding affinity of NodD for its target DNA, the way in which the nod genes are activated is discussed.

* To whom correspondence should be addressed. Tel: +86 21 64374430; Fax: +86 21 64338357; Email: gfhong@sunm.shcnc.ac.cn Present addresses: Songtao Liu, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA Weizhong Chang, Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06520-8114, USA The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors


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 Nucleic Acids ResHome page
X.-C. Chen, J. Feng, B.-H. Hou, F.-Q. Li, Q. Li, and G.-F. Hong
Modulating DNA bending affects NodD-mediated transcriptional control in Rhizobium leguminosarum
Nucleic Acids Res., May 4, 2005; 33(8): 2540 - 2548.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Feng, Q. Li, H.-L. Hu, X.-C. Chen, and G.-F. Hong
Inactivation of the nod box distal half-site allows tetrameric NodD to activate nodA transcription in an inducer-independent manner
Nucleic Acids Res., June 15, 2003; 31(12): 3143 - 3156.
[Abstract] [Full Text] [PDF]


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