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Nucleic Acids Research, 2000, Vol. 28, No. 15 2911-2914
© 2000 Oxford University Press

Universal bases for hybridization, replication and chain termination

Markus Berger, Yiqin Wu, Anthony K. Ogawa, Dustin L. McMinn, Peter G. Schultz and Floyd E. Romesberg*

Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA

Several unnatural, predominantly hydrophobic nucleobases that pack efficiently in duplex DNA without hydrogen bonding functionalities are reported to circumvent the hydrogen bonding-based specificity, both during oligonucleotide hybridization and enzymatic DNA synthesis. The reported nucleoside analogs are efficient ‘universal bases’ for hybridization, template directed DNA synthesis and chain termination. Moreover, several of the universal bases function in their biological role, hybridization or replication, with an efficiency not significantly reduced relative to their natural counterparts.

* To whom correspondence should be addressed. Tel: +1 858 784 7290; Fax: +1 858 784 7472; Email: floyd@scripps.edu


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