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Nucleic Acids Research, 2000, Vol. 28, No. 15 2915-2918
© 2000 Oxford University Press

Homogeneous assays based on deoxyribozyme catalysis

Milan N. Stojanovic*, Paloma de Prada and Donald W. Landry

Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY 10032, USA

We report herein the first homogeneous assays based on the ribonuclease activity of a deoxyribo­zyme. The previously reported deoxyribozyme was covalently modified with biotin and used to assay biotin-binding interactions through changes in fluorescence upon substrate turnover. Deoxyribozymes with fluorescence-based reporting have the potential to serve as general analytical tools.

* To whom correspondence should be addressed. Tel: +1 212 305 1152; Fax: +1 212 305 3475; Email: mns18@columbia.edu


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