Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (406K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Frohme, M.
Right arrow Articles by de Souza, A. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frohme, M.
Right arrow Articles by de Souza, A. P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 16 3100-3104
© 2000 Oxford University Press

Mapping analysis of the Xylella fastidiosa genome

Marcus Frohme1,*, Anamaria A. Camargo2, Steffen Heber1,3, Claudia Czink1, Andrew J. D. Simpson2, Jörg D. Hoheisel1 and Anete Pereira de Souza4

1Functional Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 506, D-69120 Heidelberg, Germany, 2Cancer Genetics, Ludwig Institute for Cancer Research, São Paulo, Brazil, 3Theoretical Bioinformatics, Deutsches Krebsforschungszentrum, Heidelberg, Germany and 4Genética e Evolução, Centro de Biologia Molecular e Engenharia Genética, UNICAMP, Campinas, Brazil

A cosmid library was made of the 2.7 Mb genome of the Gram-negative plant pathogenic bacterium Xylella fastidiosa and analysed by hybridisation mapping. Clones taken from the library as well as genomic restriction fragments of rarely cutting enzymes were used as probes. The latter served as a backbone for ordering the initial map contigs and thus facilitated gap closure. Also, the co-linearity of the cosmid map, and thus the eventual sequence, could be confirmed by this process. A subset of the eventual clone coverage was distributed to the Brazilian X.fastidiosa sequencing network. Data from this effort confirmed more quantitatively initial results from the hybridisation mapping that the redundancy of clone coverage ranged between 0 and 45-fold across the genome, while the average was 15-fold by experimental design. Reasons for this not unexpected fluctuation and the actual gaps are being discussed, as is the use of this effect for functional studies.

* To whom correspondence should be addressed. Tel: +49 6221 42 4678; Fax: +49 6221 42 4687; Email: m.frohme@dkfz-heidelberg.de


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genome ResHome page
M. Frohme, A. A. Camargo, C. Czink, A. Y. Matsukuma, A. J.G. Simpson, J. D. Hoheisel, and S. Verjovski-Almeida
Directed Gap Closure in Large-Scale Sequencing Projects
Genome Res., May 1, 2001; 11(5): 901 - 903.
[Abstract] [Full Text]

Home page
 Genome ResHome page
P. D. Rabinowicz
Genomics in Latin America: Reaching the Frontiers
Genome Res., March 1, 2001; 11(3): 319 - 322.
[Full Text]

Home page
 GeneticsHome page
V. Aign, U. Schulte, and J. D. Hoheisel
Hybridization-Based Mapping of Neurospora crassa Linkage Groups II and V
Genetics, March 1, 2001; 157(3): 1015 - 1020.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.