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Nucleic Acids Research, 2000, Vol. 28, No. 17 3354-3360
© 2000 Oxford University Press

The majority of human replication protein A remains complexed throughout the cell cycle

Yueh-Ming Loo and Thomas Melendy*

Department of Microbiology, School of Medicine and Biomedical Sciences, SUNY Buffalo, 138 Farber Hall, Buffalo, NY 14214-3000, USA

Replication Protein A (RPA), the replicative single-strand DNA binding protein from eukaryotic cells, is a stable heterotrimeric complex consisting of three polypeptides. Cytological studies have investigated the subcellular distribution and association characteristics of the three RPA subunits during different stages of the cell cycle with varying results. In this study, various HeLa cell fractions were subjected to separation by either immunoprecipitation or velocity sedimentation. These separations were evaluated by immunoblotting for specific RPA subunits to determine whether the RPA in these fractions retains its heterotrimeric association. Immunoprecipitation of either the large (RPA70) or middle-sized (RPA32) subunit of RPA followed by immunoblotting for the other subunits demonstrate that RPA remains complexed throughout the G1, S and G2 phases of the cell cycle. Immunoprecipitation and sedimentation separations of both the nucleosolic and chromatin-bound RPA populations from both cycling and nocodazole-blocked cells showed that the majority of RPA remains complexed under all conditions examined. Consistent with previous reports, hypotonic extracts from 293 cells were shown to contain some RPA32 not complexed with RPA70. These results indicate that in some cell types, extracts may contain small amounts of RPA32 free of RPA70; however, in HeLa cells the majority of RPA clearly remains complexed as a heterotrimer throughout the cell cycle.

* To whom correspondence should be addressed. Tel: +1 716 829 3789; Fax: +1 716 829 2158; Email: tmelendy@buffalo.edu


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S. K. Binz, Y. Lao, D. F. Lowry, and M. S. Wold
The Phosphorylation Domain of the 32-kDa Subunit of Replication Protein A (RPA) Modulates RPA-DNA Interactions: EVIDENCE FOR AN INTERSUBUNIT INTERACTION
J. Biol. Chem., September 12, 2003; 278(37): 35584 - 35591.
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