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Nucleic Acids Research, 2000, Vol. 28, No. 17 3392-3402
© 2000 Oxford University Press

The stress-activated MAP kinase Sty1/Spc1 and a 3'-regulatory element mediate UV-induced expression of the uvi15+ gene at the post-transcriptional level

Minkyu Kim, Woojin Lee, Jiyoung Park, Jae Bum Kim, Yeun Kyu Jang, Rho Hyun Seong, Soo Young Choe1 and Sang Dai Park*

School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 151-742, Republic of Korea and 1School of Life Sciences, College of Natural Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea

Exposure of Schizosaccharomyces pombe cells to UV light results in increased uvi15+ gene expression at both the mRNA and protein levels, leading to elevated cell survival. This UV-induced expression of the uvi15+ gene was reduced in {Delta}sty1 and {Delta}wis1 cells lacking the stress-activated protein kinase pathway, but not in DNA damage checkpoint mutants. To further understand the cellular mechanisms responsible for this UV-induced expression, the transcription rate and mRNA half-life were investigated. Transcription run-on assays revealed that the rate of uvi15+ transcription was increased 1.8-fold regardless of Sty1 when cells were UV irradiated. The half-life of uvi15+ mRNA was also increased 1.5-fold after UV irradiation, but it was decreased in the {Delta}sty1 background for both basal and UV-induced mRNAs, indicating that the stress-activated MAPK cascade can mediate UV-induced gene expression by increasing mRNA half-life. Deletion analyses identified a 54 nt element downstream of the distal poly(A) site, which was involved in the increased half-life of uvi15+ mRNA. These results suggest that both Sty1 and the 3'-regulatory element regulate UV-induced expression of the uvi15+ gene at the post-transcriptional level.

* To whom correspondence should be addressed. Tel: +82 2 880 6689; Fax: +82 2 887 6279; Email: sdpark@plaza.snu.ac.kr


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