Nucleic Acids Research, 2000, Vol. 28, No. 18 3462-3471
© 2000 Oxford University Press
Interaction of the U3-55k protein with U3 snoRNA is mediated by the Box B/C motif of U3 and the WD repeats of U3-55k
Department of Biochemistry and Molecular Biology and Department of Genetics, University of Georgia, Life Science Building, Athens, GA 30602, USA and 1Department of Biochemistry, University of Nijmegen, Nijmegen, The Netherlands
U3 small nucleolar RNA (snoRNA) is a member of the Box C/D family of snoRNAs which functions in ribosomal RNA processing. U3-55k is a protein that has been found to interact with U3 but not other members of the Box C/D snoRNA family. We have found that interaction of the U3-55k protein with U3 RNA in vivo is mediated by the conserved Box B/C motif which is unique to U3 snoRNA. Mutation of Box B and Box C, but not of other conserved sequence elements, disrupted interaction of U3-55k with U3 RNA. Furthermore, a fragment of U3 containing only these two conserved elements was bound by U3-55k in vivo. RNA binding assays performed in vitro indicate that Box C may be the primary determinant of the interaction. We have cloned the cDNA encoding the Xenopus laevis U3-55k protein and find strong homology to the human sequence, including six WD repeats. Deletion of WD repeats or sequences near the C-terminus of U3-55k resulted in loss of association with U3 RNA and also loss of localization of U3-55k to the nucleolus, suggesting that protein–protein interactions contribute to the localization and RNA binding of U3-55k in vivo.
* To whom correspondence should be addressed. Tel: +1 706 542 1896; Fax: +1 706 542 1752; Email: mterns@bchiris.bmb.uga.edu
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Benita, H. Kikuchi, A. D. Smith, M. Q. Zhang, D. C. Chung, and R. J. Xavier An integrative genomics approach identifies Hypoxia Inducible Factor-1 (HIF-1)-target genes that form the core response to hypoxia Nucleic Acids Res., August 1, 2009; 37(14): 4587 - 4602. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Clery, V. Senty-Segault, F. Leclerc, H. A. Raue, and C. Branlant Analysis of Sequence and Structural Features That Identify the B/C Motif of U3 Small Nucleolar RNA as the Recognition Site for the Snu13p-Rrp9p Protein Pair Mol. Cell. Biol., February 15, 2007; 27(4): 1191 - 1206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J.M. Welting, B. J. Kikkert, W. J. van Venrooij, and G. J.M. Pruijn Differential association of protein subunits with the human RNase MRP and RNase P complexes RNA, July 1, 2006; 12(7): 1373 - 1382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Granneman, J. Vogelzangs, R. Luhrmann, W. J. van Venrooij, G. J. M. Pruijn, and N. J. Watkins Role of Pre-rRNA Base Pairing and 80S Complex Formation in Subnucleolar Localization of the U3 snoRNP Mol. Cell. Biol., October 1, 2004; 24(19): 8600 - 8610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Dosil and X. R. Bustelo Functional Characterization of Pwp2, a WD Family Protein Essential for the Assembly of the 90 S Pre-ribosomal Particle J. Biol. Chem., September 3, 2004; 279(36): 37385 - 37397. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Leary, M. P. Terns, and S. Huang Components of U3 snoRNA-containing Complexes Shuttle between Nuclei and the Cytoplasm and Differentially Localize in Nucleoli: Implications for Assembly and Function Mol. Biol. Cell, January 1, 2004; 15(1): 281 - 293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. MARMIER-GOURRIER, A. CLERY, V. SENTY-SEGAULT, B. CHARPENTIER, F. SCHLOTTER, F. LECLERC, R. FOURNIER, and C. BRANLANT A structural, phylogenetic, and functional study of 15.5-kD/Snu13 protein binding on U3 small nucleolar RNA RNA, July 1, 2003; 9(7): 821 - 838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Granneman, J. E. G. Gallagher, J. Vogelzangs, W. Horstman, W. J. v. Venrooij, S. J. Baserga, and G. J. M. Pruijn The human Imp3 and Imp4 proteins form a ternary complex with hMpp10, which only interacts with the U3 snoRNA in 60-80S ribonucleoprotein complexes Nucleic Acids Res., April 1, 2003; 31(7): 1877 - 1887. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Granneman, G. J. M. Pruijn, W. Horstman, W. J. van Venrooij, R. Luhrmann, and N. J. Watkins The hU3-55K Protein Requires 15.5K Binding to the Box B/C Motif as Well as Flanking RNA Elements for Its Association with the U3 Small Nucleolar RNA in Vitro J. Biol. Chem., December 6, 2002; 277(50): 48490 - 48500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.A. GERBI, A.V. BOROVJAGIN, M. EZROKHI, and T.S. LANGE Ribosome Biogenesis: Role of Small Nucleolar RNA in Maturation of Eukaryotic rRNA Cold Spring Harb Symp Quant Biol, January 1, 2001; 66(0): 575 - 590. [Abstract] [PDF]
|
|