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Nucleic Acids Research, 2000, Vol. 28, No. 18 3462-3471
© 2000 Oxford University Press

Interaction of the U3-55k protein with U3 snoRNA is mediated by the Box B/C motif of U3 and the WD repeats of U3-55k

Andrew A. Lukowiak, Sander Granneman1, Sharon A. Mattox, Wayne A. Speckmann, Kevin Jones, Helma Pluk1, Walther J. van Venrooij1, Rebecca M. Terns and Michael P. Terns*

Department of Biochemistry and Molecular Biology and Department of Genetics, University of Georgia, Life Science Building, Athens, GA 30602, USA and 1Department of Biochemistry, University of Nijmegen, Nijmegen, The Netherlands

U3 small nucleolar RNA (snoRNA) is a member of the Box C/D family of snoRNAs which functions in ribosomal RNA processing. U3-55k is a protein that has been found to interact with U3 but not other members of the Box C/D snoRNA family. We have found that interaction of the U3-55k protein with U3 RNA in vivo is mediated by the conserved Box B/C motif which is unique to U3 snoRNA. Mutation of Box B and Box C, but not of other conserved sequence elements, disrupted interaction of U3-55k with U3 RNA. Furthermore, a fragment of U3 containing only these two conserved elements was bound by U3-55k in vivo. RNA binding assays performed in vitro indicate that Box C may be the primary determinant of the interaction. We have cloned the cDNA encoding the Xenopus laevis U3-55k protein and find strong homology to the human sequence, including six WD repeats. Deletion of WD repeats or sequences near the C-terminus of U3-55k resulted in loss of association with U3 RNA and also loss of localization of U3-55k to the nucleolus, suggesting that protein–protein interactions contribute to the localization and RNA binding of U3-55k in vivo.

* To whom correspondence should be addressed. Tel: +1 706 542 1896; Fax: +1 706 542 1752; Email: mterns@bchiris.bmb.uga.edu


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